Flow Cytometry Core

Description
The flow cytometry multi-user core facility has both North and South Campus locations. The facility is equipped with two FACScans, three FACSCaliburs, a CyAn, and a LSR II for user operated data acquisition. Users are trained to run the benchtop analyzers and billing is calculated in half hour increments.

• FACScan - single laser instrument, 488nm, capable of three fluorescent colors
• FACSCalibur - dual laser instrument with lasers at 488nm and 635nm, capable of four fluorescent colors * CyAn - a three laser, nine color instrument, with lasers at 488nm, 405nm, and 635nm
• LSR II - a four laser, sixteen color instrument, with lasers at 488nm, 532nm, 405nm, and 635nm laser *
* The Calibur and LSRII on the north campus are both equipped with a HTS, high throughput system, to acquire sample directly from a 96 or 384 well plate.

There are five cell sorters available for use in the facility, the MoFlo, and three Arias are operated by facility staff only. The AutoMacs is user operated.

• MoFlo - three laser, nine color, high speed sorter with lasers at 488nm, 633nm, and 360nm
• Aria - a five laser, eighteen color, high speed sorter with lasers at 488nm, 532nm, 405nm, 635nm
• AutoMacs – Automated benchtop magnetic cell sorter.

Training is mandatory for access to the benchtop analyzers. Training is $75 and should be scheduled when an experiment is available.


Computers and multiple software packages are available for data analysis free of charge to users.