Flow Cytometry Core

Core Description 

The Flow Cytometry Core facility is equipped with a FACScan, three FACSCaliburs, a CyAn, Canto, LSR II, and ImageStream for user operated data acquisition. A Bioreader 5000 elispot reader is also available for spot counting.

After mandatory training by facility staff, users are granted access to the lab 24/7 to run the benchtop analyzers and utilize analysis computers. Training is $100 for the Scan and Caliburs, and $125 for the Canto, CyAn, LSR II, and ImageStream. Training should be scheduled when an experiment is available.

Scan

488nm laser

3 color

$25/hr

Calibur

488nm, 635nm lasers

4 color

$25/hr

CyAn

488nm, 635nm, 405nm lasers

9 color

$45/hr

Canto

488nm, 561nm, 635nm

8 color

$45/hr

LSR

488nm, 532nm, 405nm, 635nm lasers

16 color

$52/hr

ImageStream

488nm, 634nm lasers

5 color

$52/hr

Bioreader

Elispot reader

 

$10/plate

There are five cell sorters available in the facility; facility staff operates the MoFlo and three Arias only and the AutoMacs is user operated.

MoFlo & Arias

Up to 18 color

$83/hr – first hour

 

 

$77/hr – each hour after

Computers and multiple software packages are available for data analysis free of charge to users. Flowjo is the primary analysis package and a site license is available through the IR website on MyUTSW, the campus intranet.