Flow Cytometry Core
The flow cytometry facility is located in NA2.302 and NA2.308. It is equipped with a FACScan, three FACSCaliburs, a CyAn, Canto, LSR II, and ImageStream for user-operated data acquisition. A Bioreader 5000 elispot reader is also available for spot counting.
After mandatory training by facility staff, users are granted access to lab 24/7 to run the benchtop analyzers and utilize analysis computers. Training is $100 for the Scan and Caliburs and $125 for the Canto, CyAn, LSR II, and ImageStream. Training should be scheduled when an experiment is available.
|Instrument||Laser Wavelength(s) (nm)||Colors||Hourly Fee|
|CyAn||488, 635, 405||9||$45|
|Canto||488, 561, 635||8||$45|
|LSR||488, 532, 405, 635||16||$52|
The fee for the Bioreader 5000 Elispot reader is $10/plate.
The facility has five cell sorters; facility staff operates the MoFlo and three Arias. The AutoMacs is user-operated.
|MoFlo & Arias||Up to 18 colors||$83 for first hour|
|$77 for each additional hour|
Computers and multiple software packages are available for data analysis free of charge to users. Flowjo is the primary analysis package and a site license is available through the IR website on MyUTSW, the campus intranet.