Core Facilities

The Department of Immunology provides and maintains expert Genome Sequencing, Flow Cytometry, Microarray, and Mouse Breeding technologies to the UT Southwestern Medical Center academic community.

Flow Cytometry Core Facility

The flow cytometry multi-user core facility has both North and South Campus locations. The facility is equipped with two FACScans, three FACSCaliburs, a CyAn, and a LSRII for user operated data acquisition.

Users are trained to run the benchtop analyzers and billing is calculated in half-hour increments.

• FACScan: single laser instrument, 488nm, capable of three fluorescent colors
• FACSCalibur: dual laser instrument with lasers at 488nm and 635nm, capable of four fluorescent colors*
• CyAn: a three laser, nine color instrument, with lasers at 488nm, 405nm, and 635nm
• LSRII: a four laser, 16 color instrument, with lasers at 488nm, 532nm, 405nm, and 635nm laser*

*The Calibur and LSRII on the North Campus are both equipped with a high throughput system (HTS) to acquire sample directly from a 96 or 384 well plate.

There are five cell sorters available for use in the facility:

• MoFlo – three laser, nine color, high speed sorter with lasers at 488nm, 633nm, and 360nm
• Aria – a five laser, 18 color, high speed sorter with lasers at 488nm, 532nm, 405nm, 635nm
• AutoMacs – automated benchtop magnetic cell sorter.

The MoFlo, and three Arias are operated by facility staff only. The AutoMacs is user operated.

Training is mandatory for access to the benchtop analyzers. Training is $75 and should be scheduled when an experiment is available.

Computers and multiple software packages are available for data analysis free of charge to users.

Facility Locations

Location 1
Jake L. and Nancy B. Hamon Biomedical Research Building
North Campus NA7.300
6000 Harry Hines Blvd.
Dallas, TX 75390-9093
Phone: 214-648-7325

Location 2
Cecil H. and Ida Green Biomedical Research Building
South Campus Y7.310
5323 Harry Hines Blvd.
Dallas, TX 75390-9093
Phone: 214-648-6887

Scheduling

Contact the facility you wish to use. Once you’re trained you may access the schedule.

Director

J. David Farrar, Ph.D.

Contact

Angie Mobley, Manager
Phone: 214-648-7325
angie.mobley@utsouthwestern.edu

Microarray Core Facility

The Microarray Core Facility (MCF) provides centralized access to microarray and related technology for our research community.

The MCF provides access to a variety of microarray technologies, including:

• Affymetrix GeneChip system
• Illumina BeadArray system
• Eppendorf DualChip platform
• In-house spotted microarray and proteomic arrays

Immunology provides a broad range of microarray products and services to our users, including:

• Whole genome gene expression and genotyping (using Affymetrix, Illumina, or other platforms)
• MicroRNA
• Custom DNA array printing and hybridization service
• Sample preparation (DNA, RNA) and QC (using Aligent 2100 Biolnalyzer and NanoDrop quantification)
• Microarray validation using Real-time PCR
• Microarray data analysis using a variety of bioinformatics tools including Genespring and Ingenuity(IPA)

Learn more about the microarray facility.

Facility Location

Pickens Biomedical Building
North Campus ND6.504
6000 Harry Hines Blvd.
Dallas, TX 75390-8814

Director

Edward Wakeland, Ph.D.

Associate Director

Quan-Zhen Li, M.D., Ph.D.

Contact

Jinchun Zhou, Ph.D.
Lab Manager
Phone: 214-645-6071
Fax: 214-645-6074
jinchun.zhou@utsouthwestern.edu