Publications

Publications by year

2021

  • De Nisco, N. J., Casey, A. K., Kanchwala, M., Lafrance, A. E., Coskun, F. S., Kinch, L. N., . . . & Orth, K. (2021). Manipulation of ire1-dependent mapk signaling by a vibrio agonist-antagonist effector pair. MSystems, 6(1). doi:10.1128/msystems.00872-20
  • Fernandez, J., Lopez, V., Kinch, L., Pfeifer, M. A., Gray, H., Garcia, N., . . . & Orth, K. (2021). Role of two metacaspases in development and pathogenicity of the Rice Blast fungus Magnaporthe oryzae. MBio, 12(1). doi:10.1128/mbio.03471-20
  • Stefanius, K., Servage, K., & Orth, K. (2021). Exosomes in cancer development. Current Opinion in Genetics & Development, 66, 83-92. doi:10.1016/j.gde.2020.12.018

2020

  • Chimalapati, S., de Souza Santos, M.*, Lafrance, A., Ray, A., Lee, W-R., Rivera-Cancel, G., Vale, G., Paw┼éowski, K., Mitsche, M., McDonald, J.G. , Liou, J. & Orth, K. Vibrio deploys a Type 2 secreted lipase to esterify cholesterol with host fatty acids and mediate T3SS2-mediated cell egress. eLife, In press.
  • Peng, W., Fernandez, J., Casey, A. K., Kinch, L. N., Tomchick, D. R., & Orth, K. (2020). Structures of an intact yeast v-atpase alone and in complex with bacterial effector vopq. doi:10.1101/2020.07.16.207225
  • Peng, W., Casey, A. K., Fernandez, J., Carpinone, E. M., Servage, K. A., Chen, Z., Li, Y., Tomchick, D. R., Starai, V. J., & Orth, K. (2020). A distinct inhibitory mechanism of the V-ATPase by Vibrio VopQ revealed by cryo-EM. Nature structural & molecular biology, 10.1038/s41594-020-0429-1. Advance online publication.
  • Servage, K. A., Stefanius, K., Gray, H. F., & Orth, K. (2020). Proteomic Profiling of Small Extracellular Vesicles Secreted by Human Pancreatic Cancer Cells Implicated in Cellular Transformation. Scientific reports, 10(1), 7713.

2019

  • Yang, H.*, de Souza Santos, M.*, Lee, J., Law, H.T., Chimalapati, S., Verdu, E.F., Orth, K. #, and Vallance, B.A.# A novel mouse model of enteric Vibrio parahaemolyticus infection reveals the T3SS2effector VopC plays a key role in tissue invasion and gastroenteritis. (2019) *contributed equally, #co-corresponding. mBio 17;10(6). pii: e02608-19. doi: 10.1128/mBio.02608-19.
  • Stefanius, K., Servage, K., de Souza Santos, M., Chimalapati, S., Gray, H., Kim, M., Brekken, R. & Orth, K. Pancreatic Cancer Cell Exosomes Initiate Cellular Transformation and Tumor formation. eLife, (2018) May 28;8. pii: e40226. doi: 10.7554/eLife.40226.
  • Peng, W., de Souza Santos, M., Tomchick, D. & Orth, K. Assembly mechanism of the E. coli hemolysin ClyA pore complexes. PLoS ONE (2019) May 2;14(5):e0213423. doi: 10.1371/journal.pone.0213423.
  • De Nisco, N., Neugent, M., Mull, J., Chen, L., Kuprasertkul, A., de Souza Santos, M., Palmer, K., Zimmern, P. & Orth, K. Direct detection of tissue-resident bacteria and chronic inflammation in the bladder wall of postmenopausal women with recurrent urinary tract infection. Journal of Molecular Biology (2019) Apr 17. pii: S0022-2836(19)30202-5.

2018

  • Sreelatha, A., Yee, S.S., Lopez, V.A., Park, B.C., Kinch, L., Pilch, S., Servage, K.A., Zhang, J., Jiou, J., Karasiewicz, M., M?obocka, M., Grishin, N., Orth, K, Kucharczyk, R., Paw?owski, K., Tomchick, D. R., and Tagliabracci. V.S. Protein AMPylation by an evolutionarily conserved pseudokinase. Cell. (2018) 175(3):809-821
  • Moehlman, A.T., Casey, A.K., Servage, K., Orth, K* & Krämer, H.* Regulation of the Unfolded Protein Response by BiP AMPylation protects photoreceptors from light-dependent degeneration. eLife. (2018) Jul 17:7. Pii:e38752. doi:10.7554/eLife.38752. PMID: 30015618.
  • Orth, K. My winding trail while fulfilling my love for science and family. J Biol Chem.. (2018) pii: jbc.AW118.003225. doi: 10.1074/jbc.AW118.003225
  • Chimalapati,S., de Souza Santos, M., Servage, K., De Nisco, N.J., Dalia, A.B. & Orth, K. Natural transformation of Vibrio parahaemolyticus: A rapid method to create genetic deletions. J. Bac. 2018 Jul 10;200(15). pii: e00032-18. doi: 10.1128/JB.00032-18
  • Fernandez, J. & Orth, K. Rise of a Cereal Killer: The Biology of Magnaporthe oryzae Biotrophic Growth. Trends in Microbiology (2018)
  • De Nisco, N.J., Rivera-Cancel, G. & Orth, K. The biochemistry of sensing: How enteric pathogens regulate type III secretion in response to environmental and host cues. mBio. (2018) vol. 9 no. 1 e02122-17

2017

  • Ray, A., Schwartz, N., de Souza Santos, M., Zhang, J. Orth, K & Salomon, D. Type VI secretion system MIX-effectors are targeting at both Bacterial and eukaryotic neighbors. EMBO Report. (2017) e201744226
  • Casey, A.K. & Orth, K. Enzymes involved in AMPylation and deAMPylation. Chemical Reviews. (2017) Aug 18. doi: 10.1021/acs.chemrev.7b00145.
  • Cancel-Rivera, G. & Orth, K. Biochemical basis for activation of virulence genes by bile salts in Vibrio parahaemolyticus. Gut Microbes.. (2017) Jan 27:0. doi: 10.1080/19490976.2017.1287655
  • Casey, A.K., Moelman, A., Zhang, J., Servage, K., Kramer, H. & Orth, K. Fic-mediated deAMPylation of BiP is not dependent on homo-dimerization and rescues toxic AMPylation in flies. J Biol Chem. (2017) 292 (51) pg 21193-21204)
  • de Souza Santos, M., Salomon, D. & Orth, K. T3SS effector VopL inhibits the host ROS response, promoting the intracellular survival of Vibrio parahaemolyticus. PLoS Pathog (2017) 13(6): e1006438. doi: 10.1371/journal.ppat.100643
  • Li,P., Salomon, D., Kinch, L.N., Ray, A., Dalia, A.B., Cong, Q., Ninan, L., Camilli, A., Grishin, N.V. & Orth, K. Acute Hepatopancreatic Necrosis Disease (AHPND) causing Vibrio parahaemolyticus contains a virulent Type VI Secretion System (T6SS) with anti-bacterial activities. Appl Environ Microbiol. (2017) Apr 21. pii: AEM.00737-17. doi: 10.1128/AEM.00737-17
  • De Nisco, N.J., Kanchwala, M., Li, P., Fernandez, J., Xing, C. & Orth, K. Cytotoxic Vibrio T3SS1 Rewires Host Gene Expression to Subvert Cell Death Signaling and Activate Cell Survival Networks. Sci Signal (2017) May 16;10(479). pii: eaal4501. doi: 10.1126/scisignal.aal4501
  • De Nisco, N.J., Orth, K. & VanHook, A.M., Signaling Science Podcast: Vibrio Rewires host cells. Sci Signal. 2017 May 16;10(479). pii: eaan5621. doi: 10.1126/scisignal.aan5621

2016

  • Huebinger, R.M., Stones, D.H., de Souza Santos, M., Carlson, D.L., Song, J., Vaz, D.P., Keen, E., Wolf, S.E., Orth, K. & Krachler, A. M. Targeting bacterial adherence inhibits multidrug-resistant Pseudomonas aeruginosa infection following burn injury. (2016) Scientific Reports Dec 20;6:39341. doi: 10.1038/srep39341
  • Li, P., Rivera-Cancel, G., Kinch, L.N., Salomon, D., Tomchick, D.R., Grishin, N.V. & Orth, K. Bile Salt Receptor Complex Activates a Pathogenic Type III Secretion System (2016) eLife 5:e15718.
  • Ray, A., Kinch, L.N., , de Souza Santos, M., Grishin, N.V., Orth, K. & Salomon, D. Proteomics analysis reveals previously uncharacterized virulence factors in Vibrio proteolyticus (2016) mBio 7(4):e01077-16. doi:10.1128/mBio.01077-16.

2015

  • Salomon, D., Klimko, J.A., Trudgian, D.C., Kinch, L.N., Grishin, N.V., Mirzaei, H & Orth, K. Type VI secretion system toxins horizontally shared between marine bacteria (2015) PLoS Pathogen Aug 25;11(8):e1005128. PMID 26305100.
  • Sreelatha, A., Bennett, T.L., Carpinone, E.M., O’Brien, K.M., Jordan, K.D., Burdette, D.L., Orth, K, & Starai, V.J. The Vibrio effector protein, VopQ, inhibits fusion of V-ATPase containing membranes. (2015) PNAS 112(1):100-5 PMID:25453092.

2014

  • Ham H., Woolery A.R., Tracy C., Stenesen D., Krämer H. & Orth, K. Unfolded protein response-regulated dFic reversibly AMPylates BiP during ER homeostasis. (2014) JBC 289(52):36059-69 PMID: 25395623.
  • Calder, T., Santos, M.S., Attah, V., Klimko, J., Frernandez, J., Salomon, D., Krachler, A.M., & Orth, K. Structural and regulatory mutations in Vibrio parahaemolyticus type III secretion systems display variable effects on virulence. (2014) FEMS 361(2):107-14. PMID 25288215.
  • Woolery, A.R., Yu, X., LaBaer, J.& Orth, K. AMPylation of Rho GTPases subverts multiple host signaling processes. (2014) JBC 289(47):32977-88. PMID 25301945.
  • de Souza Santos, M.S.. & Orth, K. Intracellular Vibrio parahaemolyticus Escapes the Vacuole and Establishes a Replicative Niche in the Cytosol of Epithelial Cells. (2014) mBio 9;5(5):e01506-14.
  • Yu, X., Woolery, A.R., Luong, P., Hao, Y.H., Grammel, M., Westcott, N., Park, J., Wang, J., Bian, X., Demirkan, G., Hang, H.C., Orth, K. & LaBaer, J. Click chemistry-based detection of global pathogen-host AMPylation on self-assembled human protein microarrays. (2014) Mol & Cell Proteomics 13(11):3164-76. PMID 25073739.
  • Calder, T., Kinch, L.N., Fernandez, J., Salomon, D., Grishin, N.V., & Orth, K. Vibrio type III effector VPA1380 is related to the cysteine protease domain of large bacterial toxin PLoS ONE 6;9(8):e104387 PMID 25099122.
  • Salomon, D., Klimko, & Orth, K. H-NS regulates the Vibrio parahaemolyticus type VI secretion system 1. (2014) Microbiology 160(Pt9):1867-73. PMID:24987102.
  • Salomon, D., Kinch, L.N., Trudgian, D.C., Guo, X., Klimko, J.A., Grishin, N.V., Mirzaei, H  & Orth, K. Marker for type VI secretion system effectors. PNAS USA (2014) 111(25):9271-6 PMID 24927539.
  • Lewallen,D.M., Sreelatha, A., Dharmarajan, V., Madoux, F., Chase,, F. ,Griffin, P.R., Orth, K., Hoddler, P. & Thompson, P.R. Inhibiting AMPylation: a novel screen to identify the first small molecule inhibitors of protein AMPylation. ACS Chem Biol (2014) 9(2): 433-432 PMC3944102.

2013

  • Sreelatha, A., Orth, K., & Starai, V.J. The pore-forming bacterial effector, VopQ, halts autophagic turnover. Autophagy (2013) 9(12): 2169-70. PMID:24145145.
  • Salomon D., Guo, Y., Kinch, L.N., Grishin, N.V., Mirzaei, H. & Orth, K. Effectors of animal and plant pathogens use a common domain to bind host phosphoinositides. Nature Communications (2013) 4:2973. Doi:10.1038/n,comms3973.
  • Hawley, C.A., Watson, C., Orth, K. & Krachler, A-M. High-affinity binding of bacterial fibronectin-binding proteins to host cells inhibits critical steps in wound healing. PloS ONE (2013) 8.11:e81216.
  • Sreelatha, A., Bennett,T. L., Zheng, H., Jiang, Q.X., Orth, K.*, & Starai, V.J.* Vibrio VopQ forms a gated outward rectifying channel that disrupts host ion homeostasis. PNAS USA (2013) 110(28):11559-64.
  • Altura, M.A., Heath-Heckman, E.A., Gillette, A. Kremer, A-M, Krachler, A.M. Brennean, c., Ruby, E.G., Orth, K., McFall-Ngai, M.J. (2013) The first engagement of partners in the Euprymna scolopes-Vibrio fischeri symbiosis is a two-step process initiated by a few environmental symbiont cells. Environ Microbiol. (2013) Jun 11. doi: 10.1111/1462-2920.12179 (Epub ahead of print).
  • Salomon, D. & Orth, K. What Pathogens Have Taught Us About Posttranslational Modifications (2013) Cell Host Pathogens 14:3 269-279.
  • Krachler, A-M. & Orth, K. Made to stick: Anti-adhesion therapy for bacterial infections as an alternative to conventional antimicrobials. (2013) Microbe 8:286-290.
  • Krachler, A-M. & Orth, K. Targeting the bacteria-host interface: strategies in anti-adhesion therapy (2013) Virulence 4(4): 284-94.
  • Salomon, D. & Orth, K. Lost after translation: post-translational modifications by bacterial type III effectors. (2013) Current Opinions in Microbiology 6(2):213-20.
  • Zhang, L.L & Orth, K. Virulence Determinants for Vibrio parahaemolyticus Infection. Current Opinions in Microbiology (2013) 16(1):70-7.
  • Salomon, D., Gonzalez, H., Updegraff, B.L. & Orth, K. Vibrio parahaemolyticus Type VI Secretion System 1 is Activated in Marine Conditions to Target Bacteria, and is Differentially Regulated from System 2. PLoS ONE (2013) 8:4 e61086.

2012

  • Ham, H. & Orth, K. The Role of Type III Secretion System 2 in Vibrio parahaemolyticus Pathogenicity. J Microbiol. (2012) Oct;50(5):719-25. PMID:23124738[PubMed - in process]
  • Krachler AM & Orth, K. In vitro characterization of Multivalent Adhesion Molecule 7-based inhibition of multidrug-resistant bacteria isolated from wounded military personnel. Virulence (2012) (In Press)
  • Alto, N.M. & Orth, K. Subversion of cell signaling by pathogens. In Signal Transduction Textbook. Cold Spring Harbor Press (2012).
  • Zhang, L.L, Krachler, A-M, Broberg, CA, Li, Y, Mirzaei, H, Gilpin, CJ & Orth, K. Type III effector VopC mediates invasion for Vibrio species. Cell Reports (2012) 1(5): 453-460.
  • Rahman, M., Ham, H., Liu, X., Sugiura, Y., Orth, K. and Krämer, H. Visual neurotransmission in Drosophila requires expression of dFic in glial capitate projections. Nature Neuroscience (2012) Apr 29. doi: 10.1038/nn.3102. [Epub ahead of print] PMID:22544313.
  • Krachler, A-M, Ham, H. & Orth, K. Turnabout is fair play: Use of the bacterial Multivalent Adhesion Molecule 7 as an antimicrobial agent. Virulence (2012) 1;3(1).

2011

  • Krachler AM & Orth, K. Functional characterization of the interaction between bacterial adhesin multivalent adhesion molecule 7 (MAM7) protein and its host cell ligands. J Biol Chem. (2011) Nov 11;286(45):38939-47.
  • Grammel M, Luong P, Orth, K, Hang HC. A chemical reporter for protein AMPylation. J Am Chem Soc. (2011) Nov 2;133(43):17103-5.
  • Krachler, A-M, Woolery, A.R. & Orth, K. Host-pathogen interactions: Manipulation of kinase signaling by bacterial pathogens. (2011) J. Cell Biol 195(7):1083-92.
  • Krachler AM, Ham H, Orth, K. The outer membrane adhesion factor MAM7 initiates host cell binding during infection with Gram-negative pathogens. (2011) Proc Natl Acad Sci USA. Epub June 2011.
  • Ham H., Sreelatha, A. & Orth, K. (2011) Manipulation of Host Membranes by Bacterial Effectors. Nature Reviews Microbiology 9(9):635-46.
  • Broberg, CA, Calder, TJ, & Orth, K. Vibrio parahaemolyticus cell biology and pathogenicity determinants. Microbes Infect. 2011 Nov;13(12-13):992-1001. Epub 2011 Jul 7.
  • Hao YH, Chuang T, Ball HL, Luong P, Li Y, Flores-Saaib RD, Orth, K. Characterization of a rabbit polyclonal antibody against threonine-AMPylation. (2011) J Biotechnol. 151(3):251-4.
  • Li Y, Al-Eryani R, Yerbrough ML, Orth, K, Ball HL. Characterization of AMPylation on threonine, serine, and tyrosine using mass spectrometry. (2011) J Am Soc Mass Spectrom. 22(4):752-61.
  • Krachler AM, Orth, K. Black Spot, Black Death, Black Pearl: Tales of Bacterial Effectors. Chapter in Avancées et nouvelles technologies en Toxinologie – Advances and new technologies in Toxinology (SFET, ed.). ISSN 1760 – 6004.

2010

  • Broberg CA, Zhang L, Gonzalez H, Laskowski-Arce MA, Orth, K. A Vibrio effector protein is an inositol phosphatase and disrupts host cell membrane integrity. (2010) Science. 329(5999):1660-2.
  • Luong P, Kinch LN, Brautigam CA, Grishin NV, Tomchick DR, Orth, K. Kinetic and structural insights into the mechanism of AMPylation by VopS Fic domain. (2010) J Biol Chem. 285(26):20155-63.
  • Broberg CA, Orth, K. Tipping the balance by manipulating post-translational modifications. (2010) Curr Opin Microbiol. 13(1):34-40.
  • Woolery AR, Luong P, Broberg CA, Orth, K. AMPylation: Something Old is New Again. (2010) Front Microbiol. 1:113.

2009

  • Burdette DL, Seemann J, Orth, K. Vibrio VopQ induces PI3-kinase-independent autophagy and antagonizes phagocytosis. (2009) Mol Microbiol. 73(4):639-49.
  • Kinch LN, Yarbrough ML, Orth, K, Grishin NV. Fido, a novel AMPylation domain common to fic, doc, and AvrB. (2009) PLoS One. 4(6):e5818.
  • Yarbrough ML, Orth, K. AMPylation is a new post-translational modification. (2009) Nat Chem Biol. 5(6):378-9.
  • Yarbrough ML, Li Y, Kinch LN, Grishin NV, Ball HL, Orth, K. AMPylation of Rho GTPases by Vibrio VopS disrupts effector binding and downstream signaling. (2009) Science. 323(5911):269-72.
  • Burdette DL, Yarbrough ML, Orth, K. Not without a cause: Vibrio parahaemolyticus induces acute autophagy and cell death. (2009) Autophagy. 5(1):100-2.

2008

  • Mukherjee S, Orth, K. Microbiology. A protein pupylation paradigm. (2008) Science. 322(5904):1062-3.
  • Laskowski-Arce MA, Orth, K. Acanthamoeba castellanii promotes the survival of Vibrio parahaemolyticus. (2008) Appl Environ Microbiol. 74(23):7183-8.
  • Burdette L, Yarbrough ML, Orvedahl A, Gilpin CJ, Orth, K. Vibrio parahaemolyticus orchestrates a multifaceted host cell infection by induction of autophagy, cell rounding, and then cell lysis. (2008) Proc Natl Acad Sci USA. 105(34):12497-502.
  • Mukherjee S, Orth, K. in vitro signaling by MAPK and NFkappaB pathways inhibited by Yersinia YopJ. (2008) Methods Enzymol. 438:343-53.
  • Le Negrate G, Faustin B, Welsh K, Loeffler M, Krajewska M, Hasegawa P, Mukherjee S, Orth, K, Krajewski S, Godzik A, Guiney DG, Reed JC. Salmonella secreted factor L deubiquitinase of Salmonella typhimurium inhibits NF-kappaB, suppresses IkappaBalpha ubiquitination and modulates innate immune responses. (2008) J Immunol. 180(7):5045-56.
  • Mukherjee S, Negi VS, Keitany G, Tanaka Y, Orth, K. In vitro activation of the IkappaB kinase complex by human T-cell leukemia virus type-1 Tax. (2008) J Biol Chem. 283(22):15127-33.
  • Hao YH, Wang Y, Burdette D, Mukherjee S, Keitany G, Goldsmith E, Orth, K. Structura requirements for Yersinia YopJ inhibition of MAP kinase pathways. (2008) PLoS One. 3(1):e1375.
  • Trosky JE, Liverman AD, Orth, K. Yersinia outer proteins: Yops. (2008) Cell Microbiol. 10(3):557-65.

2007 & earlier

PubMed