I am interested in how transcriptional enhancers regulate their target gene expression, using estrogen receptor α (ERα)-mediated transcription as a model system. My projects are aimed to better understand the molecular mechanisms and functions of events related to enhancer activation that leads to ERα-mediated gene regulation using molecular and cellular biology, as well as genomic and computational approaches.
Franco, H. L., Nagari, A., Malladi, V. S., Li, W., Xi, Y., Richardson, D., . . . Kraus, W. L. (2018). Enhancer transcription reveals subtype-specific gene expression programs controlling breast cancer pathogenesis. Genome Res, 28(2), 159-170. doi:10.1101/gr.226019.117
Murakami, S., Nagari, A., & Kraus, W. L. (2017). Dynamic assembly and activation of estrogen receptor alpha enhancers through coregulator switching. Genes Dev, 31(15), 1535-1548. doi:10.1101/gad.302182.117
Nagari, A., Murakami, S., Malladi, V. S., & Kraus, W. L. (2017). Computational approaches for mining GRO-Seq data to identify and characterize active enhancers. Methods Mol Biol, 1468, 121-138. doi:10.1007/978-1-4939-4035-6_10