We are a group of biophysicists, cell biologists and computational folks interested in the spatiotemporal organization of cell surface receptors, the mechanisms underlying it, and its consequences for cell signaling. We utilize light microscopy, particularly single-molecule and super-resolution imaging, to monitor molecular behavior in its native cellular environment, and we develop computer vision and mathematical modeling approaches to quantitate the observed behavior and gain insight beyond what the eye can see.
We hope that you enjoy the following 2-min Science Sketch video about the lab ...
... and these recent publications/preprints:
Vega-Lugo J, da Rocha-Azevedo B, Dasgupta A, Touret N and Jaqaman K, 2021. Analysis of conditional colocalization relationships and hierarchies from three-color microscopy images. BioRXiv preprint.
Asher WB, Geggier P, Holsey MD, Gilmore GT, Pati AK, Meszaros J, Terry DS, Mathiasen S, Kaliszewski MJ, McCauley MD, Govindaraju A, Zhou Z, Harikumar KG, Jaqaman K, Miller LJ, Smith AW, Blanchard SC and Javitch JA, 2021. Single-molecule FRET imaging of GPCR dimers in living cells. Nature Methods 18: 397-405
Jaqaman K and Ditlev JA, 2021. Biomolecular condensates in membrane receptor signaling. Current Opinion in Cell Biology 69: 48-54.
Da Rocha-Azevedo B, Lee S, Dasgupta A, Vega AR, de Oliveira LR, Kim T, Kittisopikul M, Malik Z and Jaqaman K, 2020. Heterogeneity in VEGF Receptor-2 mobility and organization on the endothelial cell surface leads to diverse models of activation by VEGF. Cell Reports 32: 108187.
Kittisopikul M, Vahabikashi A, Shimi T, Goldman RD and Jaqaman K, 2020. Adaptive multi-orientation resolution analysis of complex filamentous network images. Bioinformatics 36: 5093–5103.