Publications

Publications

2014

Chia PH, Chen B, Li P, Rosen MK, Shen K. Local F-actin Network Links Synapse Formation and Axon Branching. Cell. 2014 Jan 16;156(1-2):208-20. PMID: 24439377

Chen B, Brinkmann K, Chen Z, Pak CW, Liao Y, Shi S, Henry L, Grishin NV, Bogdan S, Rosen MKThe WAVE Regulatory Complex Links Diverse Receptors to the Actin Cytoskeleton. Cell. 2014 Jan 16;156(1-2):195-207. PMID: 24439376

2013

Zahm JA, Padrick SB, Chen Z, Pak CW, Yunus AA, Henry L, Tomchick DR, Chen Z, Rosen MKThe bacterial effector VopL organizes actin into filament-like structures. Cell. 2013 Oct 10;155(2):423-34. PMID: 24120140

Smith BA, Padrick SB, Doolittle LK, Daugherty-Clarke K, Corrêa IR Jr, Xu MQ, Goode BL, Rosen MK, Gelles J. Three-color single molecule imaging shows WASP detachment from Arp2/3 complex triggers actin filament branch formation. Elife. 2013 Sep 3;2:e01008. PMID: 24015360

Hao YH, Doyle JM, Ramanathan S, Gomez TS, Jia D, Xu M, Chen ZJ, Billadeau DD, Rosen MK, Potts PR., Regulation of WASH-dependent actin polymerization and protein trafficking by ubiquitinationCell, 2013, 152(5):1051-64. PMCID: PMC3640276

Doolittle LK, Rosen MK, Padrick SB., Measurement and analysis of in vitro actin polymerizationMethods Mol Biol, 2013, 1046:273-93. PMID: 23868594.

Doolittle LK, Rosen MK, Padrick SB., Purification of Arp2/3 Complex from Saccharomyces cerevisiaeMethods Mol Biol, 2013, 1046:251-71. PMID: 23868593.

Doolittle LK, Rosen MK, Padrick SB., Purification of native arp2/3 complex from bovine thymusMethods Mol Biol, 2013, 1046:231-50. PMID: 23868592.

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2012

Li, P., Banjade, S., Cheng, H.- C., Kim, S., Chen, B., Guo, L., Llaguno, M., Hollingsworth, J. V., King, D. S., Banani, S. F., Russo, P. S., Jiang, Q-. X., Nixon, B. T., Rosen, M.K., Phase Transitions in the Assembly of Multi-Valent Signaling Proteins, Nature, 2012, 483, 336-40. PMCID-PMC3343696.

Jia, D., Gomez, T. S., Billadeau, D. D., Rosen, M.K., Multiple Repeat Elements Within the FAM21 Tail Link the WASH Actin Regulatory Complex to the Retromer, Mol. Biol. Cell, 2012, 23(12), 2352-61. PMCID-PMC3374753.

Rizo, J., Rosen, M.K., Gardner, K. H., Enlightening Molecular Mechanisms through Study of Protein Interactions, J. Molec. Cell Biol., 2012, doi, "10.1093/jmcb/mjs036.

2011

Padrick, S. B., Doolittle, L. K., Brautigam, C. A., King, D. S., Rosen, M.K., Arp2/3 Complex is Bound and Activated by Two VCA Peptides, Proc. Natl. Acad. Sci. USA,2011, 108(33), E472-479. PMCID-PMC3158169.

Yu, B., Cheng, H.- C., Brautigam, C. A., Tomchick, D. R., Rosen, M.K., Mechanism of Actin Filament Nucleation by the Bacterial Effector VopL, Nat. Struct. Mol. Biol., 2011, 18(9), 1068-1074. PMCID-PMC3168117.

Li, P., Martins, I. R. S., Rosen, M.K., The Feasibility of Parameterizing Four-State Equilibria Using Relaxation Dispersion Measurements, J. Biomol. NMR, 2011, 51, 57-70. PMCID-PMC3229927.

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2010

Yu, B., Martins, I. R. S., Li, P., Amarasinghe, G. K., Umetani, J., Fernandez-Zapico, M., Billadeau, D. D., Machius, M., Tomchick, D. R., Rosen, M.K., Structural and Energetic Mechanisms of Cooperative Autoinhibition and Activation of Vav1, Cell, 2010, 140, 246-256. PMCID-PMC2825156.

Padrick, S. B., Rosen, M.K., Physical Mechanisms of Signal Integration by WASP Family Proteins, Ann. Rev. Biochemistry, 2010, 79, 707-735. PMCID-PMC3017724.

Westerberg, L. S., Meelu, P., Baptista, M., Adamovich, D. A., Cotta-de-Almeida, V., Seed, B., Rosen, M.K., Vandenberghe, P., Thrasher, A. J., Klein, C., Alt, F. W., Snapper, S. B., Activating WASP Mutations Associated With X-Linked Neutropenia Result in Enhanced Actin Polymerization, Altered Cytoskeletal Responses and Genomic Instability in Lymphocytes, J. Exp. Med., 2010, 207(6), 1145-1152. PMCID-PMC2882832.

Jia, D., Gomez, T. S., Metlagel, Z., Umetani, J., Otwinowski, Z., Rosen, M.K., Billadeau, D. D., WASH and WAVE Actin Regulators are Controlled by Analogous Structurally Related Complexes, Proc. Natl. Acad. Sci. USA, 2010, 107(23), 10442-10447. PMCID-PMC2890800.

Strickland, D., Yao, X., Gawlak, G., Rosen, M.K.*, Gardner, K. H.*, Sosnick, T. R,.* Rationally increasing the dynamic range of genetically-encoded, optically-controlled proteins, Nat. Methods., 2010, 7(8), 623-626. PMCID-PMC2914111.

Padrick, S. B., Deka, R. K., Chuang, J. L., Wynn, R. M., Chuang, D. T., Norgard, M. V., Rosen, M.K., Brautigam, C. A., Determination of Protein Complex Stoichiometry Through Multisignal Sedimentation Velocity Experiments, Anal. Biochem., 2010, 407(1), 89-103. PMCID-PMC3089910.

Otomo, T., Tomchick, D. R., Otomo, C., Machius, M., Rosen, M.K., Crystal structure of the Formin mDia1 in Autoinhibited Conformation, PLoSONE, 2010, 5(9), e12896. PMCID-PMC2948019.

Chen, Z., Borek, D., Padrick, S. B., Gomez, T. S., Metlagel, Z., Ismail, A., Umetani, J., Billadeau, D. D., Otwinowski, Z., Rosen, M.K., Cooperative Control Mechanisms of the Actin Regulatory WAVE Complex, Nature, 2010, 468, 533-538. PMCID-PMC3085272.

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2009

Beel, K., Cotter, M. M., Blatny, J., Bond, J., Lucas, G., Greene, F., Vanduppen, V., Leung, D., Rooney, S., Smith, O. P., Rosen, M.K., Vandenberghe, P., A large kindred with X-linked neutropenia with an I294T mutation of the Wiskott-Aldrich syndrome gene, Brit. J. Haematology, 2009, 144(1), 120-126.

Ismail, A. M., Padrick, S. B., Chen, B., Umetani, J., Rosen, M.K., The WAVE Regulatory Complex is Inhibited, Nat. Struct. Mol. Biol., 2009, 16(5), 561-563. PMCID-PMC2716658.

Vingadassalom, D., Kazlauskas. A., Skehan, B., Cheng, H.-C., Robbins, D., Magoun, L., Rosen, M.K., Saksela, K., Leong, J. M., Insulin Receptor Tyrosine Kinase Substrate links the E. coli O157, H7 actin assembly effectors Tir and EspFU during pedestal formation, Proc. Natl. Acad. Sci., 2009, 106(16), 6754-6759. PMCID-PMC2672544.

2008

Cheng, H.-C., Skehan, B. M., Campellone, K. G., Leong, J. M., Rosen, M.K., Structural Mechanism of WASP Activation by the Enterohaemorrhagic E. Coli Effector EspFU, Nature, 2008, 454, 1009-1013. PMCID-PMC2719906.

Li, P., Martins, I. R. S., Amarasinghe, G. K., Rosen, M.K., Internal Dynamics Control Activation and Activity of the Autoinhibited Vav DH Domain, Nat. Struct. Mol. Biol., 2008, 15(6), 613-618. PMCID-PMC2512264.

Leung, D. L., Otomo, C., Chory, J., Rosen, M.K., Genetically Encoded Photoswitching of Actin Assembly Through the Cdc42-WASP-Arp2/3 Complex Pathway, Proc. Natl. Acad. Sci., 2008, 105(35), 12797-12802. PMCID-PMC2525560.

Yao, X., Rosen, M.K., Gardner, K. H., Estimation of Available Free Energy in a LOV2-Ja Photoswitch, Nat. Chem. Biol., 2008, 4(8), 491-7. PMCID-PMC2597337.

Padrick, S. B., Cheng, H.-C. C., Ismail, A., Panchal, S. C., Doolittle, L. K., Kim, S., Skehan, B.M., Umetani, J., Brautigam, C. A., Leong, J. M., Rosen, M.K., Hierarchical Regulation of WASP/WAVE Proteins, Mol. Cell, 2008, 32, 426-438. PMCID-PMC2680354.

Campellone, K. G., Cheng, H.-C., Robbins, D. Siripala, A. D., McGhie, E. J., Hayward, R. D., Welch, M. D., Rosen, M.K., Koronakis, V., Leong, J. M., Repetitive N-WASP-binding elements of the enterohemorrhagic E. coli effector EspFU synergistically activate actin assembly, PLoS Pathogens, 2008, 4(10), e1000191. PMCID-PMC2567903.

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2007

Ferguson, A.D., Amezcua, C. A., Halabi, N. M., Chelliah, Y., Rosen, M.K., Ranganathan, R., Deisenhofer, J., Signal Transduction Pathway of TonB-Dependent Transporters, Proc. Natl. Acad. Sci., 2007, 104(2), 513-518. PMCID-PMC1760641.

Liverman, A. D. B., Cheng, H.-C., Trosky, J. E., Leung, D. W., Rosen, M.K., Orth, K., Arp2/3-independent assembly of actin by Vibrio type III effector VopL, Proc. Natl. Acad. Sci., 2007, 104(43), 17117-17122. PMCID-PMC2040399.

2006

Torres, E. and Rosen, M.K., Protein Tyrosine Kinase and GTPase Signals Cooperate to Phosphorylate and Activate WASP/N-WASP, J. Biol. Chem., 2006, 281(6), 3513-3520.

Leung, D. W., Morgan, D. M., Rosen, M.K., Biochemical Properties and Inhibitors of (N-) WASP, Methods Enzymol., 2006, 406, 281-296.

Tskvitaria-Fuller, I., Seth, A., Mistry, N., Gu, H, Rosen, M.K., Wülfing, C., Specific patterns of Cdc42 activity regulate distinct elements of T cell polarization, J. Immunology, 2006, 177, 1708-1720. PMCID-PMC2954656.

Seth, A., Otomo, C., Rosen, M.K., Autoinhibition Regulates the Actin Assembly Activity and Cellular Localization of the Diaphanous-Related Formins FRLa and mDia1, J. Cell Biol., 2006, 174(5), 701-713. PMCID-PMC2064313.

2005

Otomo, T., Tomchick, D. R., Otomo, C., Panchal, S. C., Machius, M., Rosen, M.K., Structural Basis of Actin Filament Nucleation and Processive Capping by a Formin Homology 2 Domain, Nature, 2005, 433, 488-494.

Leung, D. W., Rosen, M.K., The Nucleotide Switch in Cdc42 Modulates Coupling Between the GTPase-Binding and Allosteric Equilibria of WASP, Proc. Natl. Acad. Sci., 2005, 102(16), 5685-5690. PMCID-PMC556282.

Otomo, T., Otomo, C., Tomchick, D. R., Machius, M., Rosen, M.K., Structural Basis of Rho GTPase-Mediated Activation of the Formin mDia1, Mol. Cell, 2005, 18, 273-281.

Shamesh, T., Otomo, T., Rosen, M.K., Bershadsky, A. D., Kozlov, M. M., A novel mechanism of actin filament processive capping by formin, Solution of the rotation paradox, J. Cell Biol., 2005, 170(6), 889-893. PMCID-PMC2171452.

Amarasinghe, G. K. and Rosen, M.K., Acidic Region Tyrosines Provide Access Points for Allosteric Activation of the Autoinhibited Vav1 Dbl Homology Domain, Biochemistry, 2005, 44, 15257-15268.

Kreishman-Deitrick, M., Goley, E., Denison, C. Burdine, L., C., Egile, C., Li, R., Murali, N., Kodadek, T. J., Welch, M. D., Rosen, M.K., NMR Analyses of the Activation of Arp2/3 Complex by Neuronal Wiskott-Aldrich Syndrome Protein, Biochemistry, 2005, 44, 15247-15256.

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2004

Buck, M., Xu, W., Rosen, M.K., A Two-State Allosteric Model for Autoinhibition Rationalizes WASP Signal Integration and Targeting, J. Mol. Biol., 2004, 338, 271-285.

Peterson, J. R., Bickford, L. C., Morgan, D., Kim, A. S., Kirschner, M. W., Rosen, M.K., Chemical Inhibition of N-WASP by Stabilization of a Native Autoinhibited Conformation, Nat. Struct. Mol. Biol., 2004, 11, 747-755.

2003

Seth, A., Otomo, T., Yin, H., Rosen, M.K., Rational Design of Genetically Encoded FRET-Based Sensors of Cellular Cdc42 Signaling, Biochemistry, 2003, 42(15), 3997-4008.

Garrard, S. M., Capaldo, C., Gao, L., Rosen, M.K., Macara, I. G., Tomchick, D. R., Structure of Cdc42 in a complex with the GTPase-binding domain of the cell polarity protein, Par-6, EMBO J., 2003, 22(5), 1125-1133. PMCID-PMC150343.

Torres, E. and Rosen, M.K., Contingent Phosphorylation/Dephosphorylation Provides Mechanisms of Signal Integration and Molecular Memory in WASP, Molecular Cell, 2003, 11, 1215-1227.

Panchal, S. C., Kaiser, D. A., Torres, E., Pollard, T. D., Rosen, M.K., A conserved amphipathic helix in WASP/Scar proteins is essential for activation of Arp2/3 complex, Nat. Struct. Biol., 2003, 10(8), 591-598.

Kreishman-Deitrick, M., Egile, C., Hoyt, D. W., Ford, J. J., Li, R., Rosen, M.K., NMR Analysis of Methyl Groups at 100 to 500 kDa, Model Systems and Arp2/3 Complex, Biochemistry, 2003, 42, 8579-8586.

Labno, C. M., Lewis, C. M., You, D., Leung, D. W., Takesano, A., N. Kamberos, N., Seth, A., Rosen, M.K., Schwartzberg, P. L., Burkhardt, J. K., Itk functions to control actin polymerization at the immune synapse through localized activation of Cdc42 and WASP, Current Biology, 2003, 13(18), 1619-1624.

2002

Kreishman-Deitrick, M., Rosen, M.K., Ignition of a Cellular Machine, Nature Cell Biology, 2002, 4(2), E31-E33.

Chook, Y. M., Jung, A., Rosen, M.K., Blobel, G., Uncoupling Kapb2 Substrate Dissociation and Ran Binding, Biochemistry, 2002, 41, 6955-6966.

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2001

Devriendt, K., Kim, A. S., Mathijs, G., Frints, S. G. M., Schwartz, M., Van den Oord, J. J., Verhoef, G. E. G., Boogaerts, M. A., Fryns, J.-P., You, D., Rosen, M.K., Vandenberghe, P., Constitutively Activating Mutation in WASP Causes X-Linked Severe Congenital Neutropenia, Nature Genetics, 2001, 27, 313-317.

Buck, M. & Rosen, M.K., "Flipping a Switch, Science, 2001, 291, 2329-2330.

Cannon, J. L., Case, C. M., Bosco, G., Seth, A., Siminovitch, K., Rosen, M.K., Burkhardt, J. K., WASP Recruitment to the T cell-APC contact site occurs independently of Cdc42 activation, Immunity, 2001, 15(2), 249-259.

Buck, M., Xu, W., Rosen, M.K., Global disruption of the WASP autoinhibited fold on Cdc42 binding. Ligand displacement as a novel method to monitor amide hydrogen exchange, Biochemistry, 2001, 40(47), 14115-22.

2000

Kim, A. S., Kakalis, L. T., Abdul-Manan, N., Liu, G. A., Rosen, M.K., Autoinhibition and activation mechanisms of the Wiskott-Aldrich syndrome protein, Nature, 2000, 404, 151-158.

Liu, A., Hu, W., Majumdar, A., Rosen, M.K., Patel, D. J., Detection of very weak side chain-main chain hydrogen bonding interactions in medium-size 13C/15N-labeled proteins by sensitivity-enhanced NMR spectroscopy, J. Biomolec. NMR, 2000, 17, 79-82.

Liu, A., Hu, W., Majumdar, A., Rosen, M.K., Patel, D. J., NMR detection of side chain-side chain hydrogen bonding interactions in 13C/15N-labeled proteins, J. Biomolec. NMR, 2000, 17, 305-310.

Aghazadeh, B., Lowry, W.E., Huang, X.-Y., Rosen, M.K., Structural basis for relief of autoinhibition of the Dbl homology domain of proto-oncogene Vav by tyrosine phosphorylation, Cell, 2000, 102, 625-633.

Rosen, M.K., Amos, C. D., Wandless, T. J., Mechanistic Studies of affinity modulation, J. Am. Chem. Soc., 2000, 122, 11979-11982.

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1990-1999

Abdul-Manan, N., Aghazadeh, B., Liu, G. A., Majumdar, A., Ouerfelli, O., Siminovitch, K. A., Rosen, M.K., Structure of Cdc42 in Complex with the GTPase Binding Domain of the Wiskott-Aldrich Syndrome Protein, Nature, 1999, 399, 379-383.

Aghazadeh, B., Rosen, M.K., Ligand recognition by SH3 and WW domains, The role of N-alkylation in PPII helices, Chem. and Biol., 1999, 6, R241-R246.

Aghazadeh, B., Liu, G.A., Zhu, K., Zheng, Y., Rosen, M.K., Structure and Mutagenesis of the Dbl Homology Domain, Nature Structural Biology, 1998, 5, 1098-1107.

Gardner, K. H., Rosen, M.K., Kay, L. E., Global Folds of Highly Deuterated, Methyl-Protonated Proteins by Multidimensional NMR, Biochemistry, 1997, 36, 1389-1401.

Gosser, Y.Q., Nomanbhoy, T.K., Aghazadeh, B., Manor, D., Combs, C., Cerione, R.A, Rosen, M.K., C-Terminal Binding Domain of Rho-GDP Dissociation Inhibitor Directs N-Terminal Inhibitory Peptide to GTPases, Nature, 1997, 387, 814-819.

Anafi, M., Rosen, M.K., Gish, G. D., Kay, L. E., Pawson, T., A Potential SH3 Domain-binding Site in the Crk SH2 Domain, J. Biol. Chem., 1996, 271, 21365.

Rosen, M.K., Gardner, K. H., Parris, W. P., Willis, R., Pawson, T., Kay, L. E., Selective Methyl Group Protonation of Deuterated Proteins, J. Mol. Biol., 1996, 263, 627-636.

Gardner, K. H., Konrat, R., Rosen, M.K., Kay, L. E., A (H)C(CO)NH-TOCSY pulse scheme for sequential assignment of protonated methyl groups in otherwise deuterated 15N,13C labeled proteins, J. Biomol. NMR , 1996, 8, 351-356.

Rosen, M.K., Yamazaki, T., Gish, G. D., Kay, C. M., Pawson, T., Kay, L. E., Direct Demonstration of an Intramolecular SH2-Phosphotyrosine Interaction in the Crk Protein, Nature, 1995, 374, 477-479.

Yang, D., Rosen, M.K., Schreiber, S. L., The Composite FKBP12-FK506 Surface that Contacts CalcineurinJ. Am. Chem. Soc. 1993, 115, 819.

Rosen, M.K., Yang, D., Martin, P. K., Schreiber, S. L., Activation of an Inactive Immunophilin by MutagenesisJ. Am. Chem. Soc., 1993, 115, 821.

Yu, H., Rosen, M.K., Schreiber, S. L., 1H and 15N Assignments and Secondary Structure of the Src SH3 DomainFEBS Letters, 1993, 324(1), 87.

Yu, H., Rosen, M.K., Saccomano, N. A., Phillips, D., Volkmann, R. A., Schreiber, S. L., Sequential Assignment and Structure Determination of Spider Toxin w-Aga-IVBBiochemistry, 1993, 32(48), 13123-13129.

Schreiber, S. L., Liu, J., Albers, M. W., Rosen, M.K., Standaert, R. F., Wandless, T. J., Somers, P. K., Molecular Recognition of Immunophilins and Immunophilin-Ligand ComplexesTetrahedron, 1992, 48(13), 2545.

Rosen, M.K., Schreiber, S. L., Natural Products as Probes of Cellular Function, Studies of Immunophilins, Angew. Chemie, 1992, 31, 384. German version, Naturstoffe als Sonden zum Studium zellulärer Funktionen-Untersuchungen von Immunophilinen, Angew. Chemie 1992, 104, 413.

Rosen, M.K., Belshaw, P. J., Alberg, D. G., Schreiber, S. L., The Conformation of Cyclosporin A Bound to Cyclophilin is Altered (Once Again) Following Binding to Calcineurin, An Analysis of Receptor-Ligand-Receptor InteractionsBio. Med. Chem. Lett., 1992, 2(7), 747.

Yu, H., Rosen, M.K., Shin, T. B., Seidel-Dugan, C., Brugge, J. S., Schreiber, S. L., Solution Structure of the SH3 Domain of Src and Identification of its Ligand-Binding SiteScience, 1992, 258, 1665.

Rosen, M.K., Michnick, S. W., Karplus, M., and Schreiber, S. L., Proton and Nitrogen Sequential Assignments and Secondary Structure Determination of the Human FK506 and Rapamycin Binding ProteinBiochemistry, 1991, 30, 4774.

Wandless, T. J., Michnick, S. W., Rosen, M.K., Karplus, M., Schreiber, S. L., FK506 and Rapamycin binding to FKBP, Common Elements in Immunophilin-Ligand Complexation,  J. Am. Chem. Soc., 1991, 113, 2339.

Michnick, S. W., Rosen, M.K., Wandless, T. J., Karplus, M., Schreiber, S. L., Solution Structure of FKBP, a Rotamase Enzyme and Receptor for FK506 and RapamycinScience, 1991, 252, 836.

Rosen, M.K., Albers, M. W., Schreiber, S. L., Rapamycin Sensitivity in Saccharomyces cerevisiae is Mediated by a Peptidyl-Prolyl cis-trans Isomerase Related to Human FK506-Binding Protein ChemTracts, Organic Chemistry, 1991, 4, 202; ChemTracts, Biochemistry and Molecular Biology, 1991, 2, 204.

Rosen, M.K., Michnick, S. W., Wandless, T. J., Schreiber, S. L., Study of Receptor-Ligand Interactions through Receptor Labeling and Isotope-Edited NMR J. Org. Chem., 1991, 56(22), 6262.

Schreiber, S. L., Liu, J., Albers, M. W., Karmacharya, R., Koh, E., Martin, P. K., Rosen, M.K., Standaert, R. F. Wandless, T. J., Immunophilin-Ligand Complexes as Probes of Intracellular Signaling Pathways, Transplantation Proc., 1991, 23, 2851.

Rosen, M.K., Standaert, R. F., Galat, A., Nakatsuka, M., Schreiber, S. L., Inhibition of FKBP Rotamase Activity by Immunosuppressant FK506, Twisted Amide Surrogate, Science 1990, 248, 863.

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