About Us

Nicole Nischan, Ph.D.

Education

Ph.D. – Freie Universität Berlin, Germany, in Natural Sciences, summa cum laude, 2015

Diplom (B.Sc. + M.Sc.) – Technical University Dresden, Germany, in Chemistry, 2009

 

Research

 Pertussis toxin is the major virulence factor of Bordetella pertussis, or whooping cough, that binds to surface receptors of host cells, gets internalized and interferes with cell signaling by ADP-ribosylating Gi-proteins. Thereby, pertussis toxin suppresses the innate and acquired immune response of infected individuals, thereby promoting infection. While the systemic effects of pertussis toxin downstream signaling have been extensively studied, limited knowledge is available about the cellular binding and uptake of PT.

I aim to identify the glycosylated cell-surface binding partners responsible for the cellular uptake of pertussis toxin. For this, I am using advanced mass spectrometry, biochemistry, cell culture, chemoselective reactions as well as chemical biology techniques. Among those, I am using a photocrosslinking tool, a diazirine-modified N-acetyl mannosamine (ManNDAz) that cells metabolically incorporate in their glycans. Upon light irradiation, the resulting sialic acids on the endogenous glycans forms covalent bonds with nearby molecules, allowing the capture of binding events too weak to study by immunoprecipitation methods.

These findings will lead identify both protein and glycan components of cell-surface receptors of PT, enabling to develop therapeutic agents to perturb the cell entry of PT.

 

Selected Publications

 

Hoffmann E, Streichert K, Nischan N, Seitz C, Brunner T, Schwagerus S, Hackenberger CPR, Rubini M. Stabilization of bacterially expressed Erythropoietin by single site-specific introduction of short branched PEG chains at naturally occurring glycosylation sites. Mol. Biosyst. (2016), published online 11 Jan 2016. Doi: 10.1039/C5MB00857C

http://dx.doi.org/10.1039/C5MB00857C

           

Wands AM, Fujita A, McCombs JE, Cervin J, Dedic B, Rodriguez AC, Nischan N, Bond MR, Mettlen M, Trudgian DC, Lemoff A, Quiding-Jarbrink M, Gustavsson B, Steentoft C, Clausen H, Mirzaei H, Teneberg S, Yrlid U, Kohler JJ. Fucosylation and protein glycosylation create functional receptors for cholera toxin. eLIFE. (2015) Doi: 10.7554/eLife.09545

http://elifesciences.org/content/early/2015/10/31/eLife.09545

 

Pham ND, Fermaintt CS, Rodriguez AC, McCombs JE, Nischan N, Kohler JJ. Cellular metabolism of unnatural sialic acid precursors. Glycoconj. J. (2015), 32, 515-529.

http://www.jbc.org/content/early/2015/08/03/jbc.M115.667006

 

Nischan N, Herce HD, Natale F, Bohlke N, Budisa N, Cardoso MC and Hackenberger CPR. Covalent Attachment of Cyclic TAT Peptides to GFP Results in Protein Delivery into Live Cells with Immediate Bioavailability. Ang. Chem. Int. Ed. (2015) 54: 1950-53.

http://onlinelibrary.wiley.com/doi/10.1002/anie.201410006/abstract

 

Nischan N, Hackenberger CPR. Site-specific PEGylation of proteins: recent developments. J. Org. Chem. (2014) 79: 10727-33.

http://pubs.acs.org/doi/abs/10.1021/jo502136n

           

Tronnier A, Nischan N, Metz S, Wagenblast G, Münster I, Strassner T. Phosphorescent C∧C* Cyclometalated PtII Dibenzofuranyl-NHC Complexes – An Auxiliary Ligand Study. Berichte der deutschen chemischen Gesellschaft (2014), 256-264.

http://onlinelibrary.wiley.com/doi/10.1002/ejic.201301398/abstract

 

Nischan N, Chakrabarti A, Serwa RA, Bovee-Geurts PHM, Brock R and Hackenberger CPR. Stabilization of Peptides for Intracellular Applications by Phosphoramidate-Linked Polyethylene Glycol Chains. Ang. Chem. Int. Ed. (2013) 52: 11920-24.

 

Tronnier A, Nischan N, Strassner T. CC*-cyclometalated platinum(II) complexes with trifluoromethyl-acetylacetonate ligands - Synthesis and electronic effects. J. Organomet Chem. (2013), 730, 37-43.

http://www.sciencedirect.com/science/article/pii/S0022328X12004998

 

 

Related

http://www.ncbi.nlm.nih.gov/pubmed/?term=nicole+nischan

http://www.researchgate.net/profile/Nicole_Nischan

https://www.linkedin.com/pub/nicole-nischan/8b/633/528

https://www.youtube.com/watch?v=d68P_c4EfaU