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Cell Biology & Imaging Core

Mission of the Physiology Core

Cell Biology & Imaging Core sample image
  • to provide high quality histopathologic and scientific support for research projects by localizing proteins and/or mRNAs in tissues and cultured cells
  • to offer pathology support.

The Core maintains a library of wild-type murine embryos and adult tissues embedded in paraffin for use by O'Brien Center investigators. The lab also maintains bright-field, dark-field, phase-contrast, epifluorescence, and confocal microscopes.

Physiology Core Services

Request Services:

  • Imaging of rodent kidney and heart including functional MRI
    • Investigators are responsible for time reserved on the magnet.
      • Apparent diffusion coefficient (fibrosis)
      • Arterial spin labeling (blood flow)
      • BOLD (oxygenation) Na MRS
  • Pathology
    • Processing of tissues for routine paraffin embedding
    • Detailed paraffin and cryo-microtomy
    • Standard histologic stains and special stains
    • Immunohistochemistry and in situ hybridization
    • Kidney pathology
    • Single tubule isolation and immunocytochemistry
    • Laser microdissection

Schedule Equipment:

  • Zeiss LSM 880 Meta Confocal Microscope
    • Training provided for the following:
      • Image Fixed Cells
      • Image Live Cells
      • Zen Software
Stained tissue slices