Human retinal pigment epithelial cells migrating through a three-dimensional collagen extracellular matrix. Vimentin, a powerful marker for the epithelial to mesenchymal transition in cancer, is shown in magenta, and microtubules in green.

The Microscopy Innovation Lab is the product of a highly collaborative research environment at UT Southwestern, and many of the technologies were originally developed in the Danuser and Fiolka Labs.  Below is a list of publications that describe these technologies, and their applications.

  • Robust and automated detection of subcellular morphological motifs in 3D microscopy images. Meghan K Driscoll, Erik S Welf, Andrew R Jamieson, Kevin M Dean, Tadamoto Isogai, Reto Fiolka, Gaudenz Danuser. Nature Methods
  • An image-based assay to quantify changes in proliferation and viability upon drug treatment in 3D microenvironments Authors Vasanth S Murali, Bo-Jui Chang, Reto Fiolka, Gaudenz Danuser, Murat Can Cobanoglu, Erik S Welf. BMC Cancer.
  • Nuclear positioning facilitates amoeboid migration along the path of least resistance. Jörg Renkawitz, Aglaja Kopf, Julian Stopp, Ingrid de Vries, Meghan K Driscoll, Jack Merrin, Robert Hauschild, Erik S Welf, Gaudenz Danuser, Reto Fiolka, Michael Sixt. Nature.
  • Enhanced Dendritic Actin Network Formation in Extended Lamellipodia Drives Proliferation in Growth-Challenged Rac1P29S Melanoma Cells. Ashwathi S Mohan, Kevin M Dean, Tadamoto Isogai, Stacy Y Kasitinon, Vasanth S Murali, Philippe Roudot, Alex Groisman, Dana K Reed, Erik S Welf, Sangyoon J Han, Jungsik Noh, Gaudenz Danuser. Developmental Cell.
  • Universal light-sheet generation with field synthesis. Bo-Jui Chang, Mark Kittisopikul, Kevin M Dean, Philippe Roudot, Erik S Welf, Reto Fiolka. Nature Methods.
  • Lossless Three-Dimensional Parallelization in Digitally Scanned Light-Sheet Fluorescence Microscopy. Kevin M Dean, Reto Fiolka. Scientific Reports.
  • Imaging subcellular dynamics with fast and light-efficient volumetrically parallelized microscopy. Kevin M Dean, Philippe Roudot, Erik S Welf, Theresa Pohlkamp, Gerard Garrelts, Joachim Herz, Reto Fiolka. Optica.
  • A bright cyan-excitable orange fluorescent protein facilitates dual-emission microscopy and enhances bioluminescence imaging in vivo. Jun Chu, Younghee Oh, Alex Sens, Niloufar Ataie, Hod Dana, John J Macklin, Tal Laviv, Erik S Welf, Kevin M Dean, Feijie Zhang, Benjamin B Kim, Clement Tran Tang, Michelle Hu, Michelle A Baird, Michael W Davidson, Mark A Kay, Reto Fiolka, Ryohei Yasuda, Douglas S Kim, Ho-Leung Ng, Michael Z Lin. Nature Biotechnology.
  • Diagonally scanned light-sheet microscopy for fast volumetric imaging of adherent cells. Kevin M Dean, Philippe Roudot, Carlos R Reis, Erik S Welf, Marcel Mettlen, Reto Fiolka. Biophysical Journal.
  • Quantitative multiscale cell imaging in controlled 3D microenvironments. Erik S Welf, Meghan K Driscoll, Kevin M Dean, Claudia Schäfer, Jun Chu, Michael W Davidson, Michael Z Lin, Gaudenz Danuser, Reto Fiolka. Developmental Cell.
  • Deconvolution-free subcellular imaging with axially swept light sheet microscopy. Kevin M Dean, Philippe Roudot, Erik S Welf, Gaudenz Danuser, Reto Fiolka. Biophysical Journal.
  • Uniform and scalable light-sheets generated by extended focusing. Kevin M Dean, Reto Fiolka. Optics Express.