Stem Cell Niche
The hematopoietic stem cell niche from a mouse bone marrow specimen. 3D segmented and rendered view of c-kit+ stem/progenitor cells (magenta), nerve fibers (green), arterioles (orange), and blood-vessels (gray). c-kit+ cells are in contact with nerve fibers, as well as fenestrations in the wall of the sinusoids.
Expansion Microscopy + ct-ASLM. By combining expansion microscopy with the high-resolution version of ct-ASLM, we achieve ~80 nm isotropic (e.g., XYZ) resolution and molecular specificity through intact portions of brain tissue.
Whole Brain Imaging
Whole Brain Imaging, with Synaptic-Level Resolution. An XY view of a section 2.5 mm from the top surface of a Thy1-eGFP PEGASOS cleared brain shows both large-scale neuroanatomy and individual synaptic spines.
Glomeruli imaged throughout an intact mouse kidney. Volume rendering of mouse kidney labeled with Flk1-GFP, with single cell resolution throughout millimeters of tissue. Clusters of cells form glomeruli, which are the most fundamental filteration units of the kidney.
A Migrating Breast Cancer Cell
A migrating MDA-MB-231 breast cancer cell labeled with EGFP-Tractin. 1500 Z-stacks were acquired without signs of photobleaching or phototoxicity, comprising 306,000 individual images.
3D Particle Tracking of Clathrin-Associated Structures in Caco-2 Human Colon Carcinoma Cells at a 3.5 Hz Volumetric Acquisition Rate. The vast majority of tracks show a confined displacement at the membrane and thus exhibit a low diffusion coefficient (red colored tracks). Rare events of active translocations can be detected with a maximum velocity 4.5 μm/s (shown in blue).
Human Bronchial Epithelial Cells
Cancerous human bronchial epithelial cells expressing a GFP-based probe that allows one to visualize the actin cytoskeleton.