The Microscopy Innovation Lab performs cutting-edge light-sheet and laser-scanning microscopy to accelerate biomedical discovery. Specimens range from living animals, to isolated cells, or chemically cleared tissue specimens. Below you will find a few examples of the data that has been collected in the Microscopy Innovation Lab.

Stem Cell Niche

The hematopoietic stem cell niche from a mouse bone marrow specimen.  3D segmented and rendered view of c-kit+ stem/progenitor cells (magenta), nerve fibers (green), arterioles (orange), and blood-vessels (gray).  c-kit+ cells are in contact with nerve fibers, as well as fenestrations in the wall of the sinusoids.

Expansion Microscopy

Expansion Microscopy + ct-ASLM.  By combining expansion microscopy with the high-resolution version of ct-ASLM, we achieve ~80 nm isotropic (e.g., XYZ) resolution and molecular specificity through intact portions of brain tissue.  

Whole Brain Imaging

Whole Brain Imaging, with Synaptic-Level Resolution.  An XY view of a section 2.5 mm from the top surface of a Thy1-eGFP PEGASOS cleared brain shows both large-scale neuroanatomy and individual synaptic spines.

Kidney Imaging

Glomeruli imaged throughout an intact mouse kidney.  Volume rendering of mouse kidney labeled with Flk1-GFP, with single cell resolution throughout millimeters of tissue.  Clusters of cells form glomeruli, which are the most fundamental filteration units of the kidney.

A Migrating Breast Cancer Cell

A migrating MDA-MB-231 breast cancer cell labeled with EGFP-Tractin. 1500 Z-stacks were acquired without signs of photobleaching or phototoxicity, comprising 306,000 individual images.

Clathrin-Mediated Endocytosis

3D Particle Tracking of Clathrin-Associated Structures in Caco-2 Human Colon Carcinoma Cells at a 3.5 Hz Volumetric Acquisition Rate. The vast majority of tracks show a confined displacement at the membrane and thus exhibit a low diffusion coefficient (red colored tracks). Rare events of active translocations can be detected with a maximum velocity 4.5 μm/s (shown in blue).

Human Bronchial Epithelial Cells

Cancerous human bronchial epithelial cells expressing a GFP-based probe that allows one to visualize the actin cytoskeleton.