Microscopy Innovation Lab Technology
- Cleared Tissue Axially Swept Light-Sheet Microscope (ctASLM) for single-cell biology throughout millimeters of tissues. Capable of imaging in any clearing solvent (PEGASOS, BABB, CLARITY, etc.), and roughly 40x faster imaging than a confocal. Two versions exist:
- High-NA - Isotropic resolution of ~350 nm in BABB and ~450 nm in water. Field of view of 328 microns in water, and 270 microns in BABB. 488/560/640 nm illumination sources.
- Medium-NA – Isotropic resolution of ~700 nm. Field of view 870 microns. 405/488/560/640 nm illumination sources.
- Oblique Plane Microscope (OPM). Designed for imaging on optical glass substrates High-speed (~400 frames per second) light-sheet microscope with ~270 and ~650 nm lateral and axial resolution, respectively. 488/560 nm illumination sources.
- Spectral Imaging System. Anticipated 8 color live-cell imaging in total internal reflection and epi-fluorescence geometries via spectral unmixing. Under development.
- 2 & 3-photon laser scanning microscope with adaptive optics. Anticipated intravital imaging at depths beyond 1 mm. Under development.
- Additional live-cell light-sheet microscopy equipment is available on a limited basis, including a high-resolution Axially Swept Light-Sheet Microscope, the Micro-Environmental Selective Plane Illumination Microscope, Field Synthesis (e.g., Lattice Light-Sheet Microscopy), and a Multi-View Light-Sheet Microscope optimized for imaging developing embryos and zebrafish.
Additionally, the Microscopy Innovation Lab has a high-performance computer for data visualization and and processing, equipped with 1.048 TB of RAM, 28 CPU cores, and a nVidia Quadro GPU with 24 GB of on-board memory. Software on this computer includes Amira, Arivisl, Imaris, Fiji, and more.