Lipid Modification

Although it is still debating where the initial autophagosome membrane comes from, it is a consensus that PI3P generation is the first noticeable and essential change on the initiating membrane structures. The class III phosphatidylinositol 3-kinase (PI3KC3) has an evolutionarily conserved role in producing PI3P on autophagosome membrane. We are the first group to purify human PI3KC3 holocomplex, which contains seven stoichiometric subunits. Among these subunits, we identified Barkor (Beclin 1 interacting Autophagy Related Key regulator, also known as Atg14 or Atg14L (Like)) as the autophagy specific targeting and regulatory factor of PI3KC3.

Lipid Modification
Lipid Modification

Representative papers:

  1. Sun Q, Fan W, Chen K, Ding X, Chen S, Zhong Q. Identification of Barkor as a Mammalian Autophagy-Specific Factor for Beclin 1 and Class III Phosphatidyl-inositol 3-Kinase. Proc Natl Acad Sci U S A. 2008 Dec 9;105(49):19211-6.
  2. Sun Q, Westphal W, Wong K, Tan I, Zhong Q. Rubicon controls endosome maturation as a Rab7 effector. Proc Natl Acad Sci U S A. 2010 Nov 9; 107(45):19338-43.
  3. Kim J, Kim Y, Fang C, Russell R, Kim J, Fan W, Liu R, Zhong Q, Guan KL. Differential regulation of distinct Vps34 complexes by AMPK in nutrient stress and autophagy. Cell, 2013 Jan 17;152(1), 290-303.
  4. Nazarko V and Zhong Q. ULK1 phosphorylates Beclin 1 in autophagy. Nat Cell Biol, 2013, July 1;15(7):727-8.