Publications

Publications for Dr. Arun Radhakrishnan

2012 - Present  (UT Southwestern)

Brown, M.S., Radhakrishnan, A., and Goldstein, J.L. (2018). Retrospective on cholesterol homeostasis: The central role of Scap.  Annu. Rev. Biochem. 87, 783-807.  

Infante, R.E., and Radhakrishnan, A. (2017). Continuous transport of a small fraction of plasma membrane cholesterol to endoplasmic reticulum regulates total cellular cholesterol.  eLife 2017; 6:e25466.

Gao, Y., Zhou, Y., Goldstein, J.L., Brown, M.S., and Radhakrishnan, A. (2017).  Cholesterol-induced conformational changes in the sterol-sensing domain of the Scap protein suggest feedback mechanism to control cholesterol synthesis.  J. Biol. Chem. 292, 8729-8737.

Chakrabarti, R.S., Ingham, S.A., Kozlitina, J., Gay, A., Cohen, J.C., Radhakrishnan, A., and Hobbs, H.H. (2017). Variability of cholesterol accessibility in human red blood cells measured using a bacterial cholesterol-binding toxin.  eLife 2017; 6:e23355.

Zhang, Y., Lee, K.M., Kinch, L.N., Clark, L., Grishin, N.V., Rosenbaum, D.M., Brown, M.S., Goldstein, J.L., and Radhakrishnan, A. (2016).  Direct demonstration that Loop1 of Scap binds to Loop7:  A crucial event in cholesterol homeostasis.  J. Biol. Chem. 291, 12888-12896.

Ren, R., Zhou, X., He, Y., Ke, M., Wu, J., Liu, X., Yan, C., Wu, Y., Gong, X., Lei, X., Yan, F.S., Radhakrishnan, A., and Yan, N. (2015).  Crystal structure of a mycobacterial Insig homolog provides insight into how these sensors monitor sterol levels.  Science 349, 187-191.

Gay, A., Rye, D., and Radhakrishnan, A. (2015). Switch-like responses of two cholesterol sensors do not require protein oligomerization in membranes.  Biophys. J. 108, 1459-1469.

Das, A., Brown, M.S., Anderson, D.D., Goldstein, J.L., and Radhakrishnan, A. (2014).Three pools of plasma membrane cholesterol and their relation to cholesterol homeostasis.  eLife 2014; 3:e02882.

Das, A., Goldstein, J.L., Anderson, D.D., Brown, M.S., and Radhakrishnan, A. (2013). Use of mutant 125I-Perfringolysin O to probe transport and organization of cholesterol in membranes of animal cells. Proc. Natl. Acad. Sci. USA 110, 10580-10585.

2008 - 2010  (Weill Cornell Medical College)

Sokolov, A., and Radhakrishnan, A. (2010). Accessibility of cholesterol in endoplasmic reticulum membranes and activation of SREBP-2 switch abruptly at a common cholesterol threshold. J. Biol. Chem. 285, 29480-29490.

See Commentary:  Wattenberg, B. (2010).  Hyperactive cholesterol breaks free to signal enough is enough.  ASBMB Lipid Corner, 15 July 2010. 

Radhakrishnan, A. (2010).  Phase separations in binary and ternary cholesterol-phospholipid mixtures. Biophys. J. 98, L41-L43.

2003 - 2008  (Postdoctoral fellow with Drs. Mike Brown and Joe Goldstein at UT Southwestern Medical Center)

Abi-Mosleh, L., Infante, R.E., Radhakrishnan, A., Goldstein, J.L., and Brown, M.S. (2009).Cyclodextrin overcomes deficient lysosome-to-endoplasmic reticulum transport of cholesterol in Niemann-Pick type C cells. Proc. Natl. Acad. Sci. USA. 106, 19316-19321.

Radhakrishnan, A., Sun, L., Espenshade, P.J., Goldstein, J.L., and Brown, M.S. (2009).The SREBP Pathway: Gene regulation through sterol sensing and gated protein trafficking.  In Handbook of Cell Signaling, 2ndedition (edited by R. Bradshaw and E. Dennis). Academic Press Inc., New York, Chapter 298, 2505-2510.

Radhakrishnan, A., Goldstein, J.L., McDonald, J.G., and Brown, M.S. (2008). Switch-like control of SREBP-2 transport triggered by small changes in ER cholesterol. Cell Metabolism 8, 512-521.

See Preview: Hampton, R.Y. (2008). A cholesterol toggle switch.  Cell Metabolism 8, 451-453.

Infante, R.E., Wang, M.L., Radhakrishnan, A., Kwon, H.J., Brown, M.S., and Goldstein, J.L. (2008). NPC2 facilitates bidirectional transfer of cholesterol between NPC1 and lipid bilayers, a step in cholesterol egress from lysosomes. Proc. Natl. Acad. Sci. USA 105, 15287-15292.

See Commentary:  Subramanian, K., and Balch, W.E. (2008). NPC1/NPC2 function as a tag team duo to mobilize cholesterol. Proc. Natl. Acad. Sci. USA 105, 15223-15224.

McConnell, H., and Radhakrishnan, A. (2008). Molecular motion at the critical point in lipid membranes. Biophys. J. 95, L22-L24.

Infante, R.E., Radhakrishnan, A., Abi-Mosleh, L., Kinch, L.N., Wang, M.L., Grishin, N.V., Goldstein, J.L., and Brown, M.S. (2008). Purified NPC1 protein: II. Localization of sterol binding to a 240-amino acid soluble luminal loop. J. Biol. Chem. 283, 1064-1075.

Infante, R.E., Abi-Mosleh, L., Radhakrishnan, A., Dale, J.D., Brown, M.S., and Goldstein, J.L. (2008). Purified NPC1 protein: I. Binding of cholesterol and oxysterols to a 1278-amino acid membrane protein. J. Biol. Chem. 283, 1052-1063.

Radhakrishnan, A., Ikeda, Y., Kwon, H.J., Brown, M.S., and Goldstein, J.L. (2007). Sterol-regulated transport of SREBPs from endoplasmic reticulum to Golgi:I. Oxysterols block transport by binding to Insigs. Proc. Natl. Acad. Sci. USA 104, 6511-6518. (Feature Article)

See Commentary:  Schekman, R. (2007). How sterols regulate protein sorting and traffic. Proc. Natl. Acad. Sci. USA 104, 6496-6497.  

Radhakrishnan, A., and McConnell, H. (2007). Composition fluctuations, chemical exchange and nuclear relaxation in membranes containing cholesterol. J. Chem. Phys. 126, 185101.

McConnell H., and Radhakrishnan, A. (2006). Theory of the deuterium NMR of sterol-phospholipid membranes. Proc. Natl. Acad. Sci. USA 103, 1184-1189.

Radhakrishnan, A., and McConnell H. (2005). Condensed complexes in vesicles containing cholesterol and phospholipids. Proc. Natl. Acad. Sci. USA 102, 12662-12666.

Feramisco, J.D., Radhakrishnan, A., Ikeda, Y., Reitz, J., Brown, M.S. and Goldstein, J.L. (2005). Intramembrane aspartic acid in Scap protein governs cholesterol-mediated conformational change. Proc. Natl. Acad. Sci. USA 102, 3242-3247.  

Radhakrishnan, A., Sun, L., Kwon, H.J., Brown, M.S., and Goldstein, J.L. (2004). Direct binding of cholesterol to the purified membrane region of SCAP: Mechanism for a sterol-sensing domain. Mol. Cell 15, 259-268.

1997 - 2002  (Graduate student with Dr. Harden McConnell at Stanford University)

McConnell, H.M., and Radhakrishnan, A. (2003). Condensed complexes of cholesterol and phospholipid. BBA Reviews 1610, 159-173.

Radhakrishnan, A., and McConnell, H.M. (2002). Critical points in charged membranes containing cholesterol. Proc. Natl. Acad. Sci. USA 99, 13391-13396.

Okonogi, T.M., Radhakrishnan, A., and McConnell, H.M. (2002).Two fatty acids can replace one phospholipid in condensed complexes with cholesterol.  Biochim. Biophys. Acta 1564, 1-4.

Radhakrishnan, A., and McConnell, H.M. (2002). Thermal dissociation of condensed complexes of cholesterol and phospholipid. J. Phys. Chem. B 106, 4755-4762.

Radhakrishnan, A., Li, X., Brown, R.E., and McConnell, H.M. 2001. Stoichiometry of cholesterol-sphingomyelin condensed complexes in monolayers. Biochim. Biophys. Acta 1511, 1-6.

Radhakrishnan, A., Anderson, T.G., and McConnell, H.M. (2000). Condensed complexes, rafts and the chemical activity of cholesterol. Proc. Natl. Acad. Sci. USA 97, 12422-12427.

Keller, S.L., Radhakrishnan, A., and McConnell, H.M. (2000). Saturated phospholipids with high melting temperatures form complexes with cholesterol in monolayers. J. Phys. Chem. B 104, 7522-7527.

Radhakrishnan, A., and McConnell, H.M. (2000). Chemical activity of cholesterol in membranes. Biochemistry39, 8119-8124.

Radhakrishnan, A., and McConnell, H.M. (2000). Electric field effect on cholesterol-phospholipid complexes. Proc. Natl. Acad. Sci. USA 97, 1073-1078.

Radhakrishnan, A., and McConnell, H.M. (1999). Condensed complexes of cholesterol and phospholipids. Biophys. J. 77, 1507-1517.

Radhakrishnan, A., and McConnell, H.M. (1999).  Cholesterol-phospholipid complexes in membranes. J. Am. Chem. Soc. 121, 486-487.

1996 - 1997  (Undergraduate researcher with Dr. Richard Mathies at UC Berkeley)

Scherer, J.R., Kheterpal, I. Radhakrishnan, A., Ja, W.W., and Mathies, R.A. (1999). Ultra-high throughput rotary capillary array electrophoresis scanner for fluorescent DNA sequencing and analysis. Electrophoresis 20, 1508-1517.

Kheterpal, I., Scherer, J.R., Clark, S.M., Radhakrishnan, A., Ju, J., Ginther, C.L., Sensabaugh, G.F., and Mathies, R.A. (1996). DNA sequencing using a four-color confocal fluorescence capillary array scanner. Electrophoresis17, 1852-1859.

Kheterpal, I., Ju, J., Radhakrishnan, A., Brandt, G.S., Ginther, C.L., Clark, S.M., Scherer, J.R., Sensabaugh, G.F., and Mathies, R.A. (1996). DNA sequencing using four-color capillary array electrophoresis and energy transfer primers. Ultrasensitive Biochemical Diagnostics – Proceedings of the International Society for Optical Engineering-SPIE 2680, 204-213.