High-throughput strategies for deconstructing cell fate determination pathways in cancer

Cell fate diagram

Decades of genetically based investigation into the molecular underpinnings of embryonic development have identified highly conserved signaling systems that coordinate cell fate decision-making in metazoans. The potent ability of these systems when corrupted to transform stem cells into cancer initiating cells have made them high priority anti-cancer therapeutic targets.

We apply large molecular library screening in cultured cell models for several of these signaling processes, including those controlled by the Wnt, Hedgehog, and Notch molecules, in order to identify strategies for manipulating them in the context of cancerous cells.