Hormonal effect in mouse models

Stained cells
Histology of lungs from our mouse model of Pseudomonas aeruginosa infection.

We use murine models of acute and chronic infection with Pseudomonas aeruginosa in wild-type and CFTR-null mice to determine the impact of sex hormones on inflammation and bacterial clearance.

Male and female mice, both intact and ovariectomized, undergo hormone manipulation to determine specific receptor influences and target pathways. We employ a array of techniques such as immunohistochemistry, flow cytometry, and RT-PCR.