Increased imaging speeds in 3D fluorescence microscopy through parallelization

Rendering of cultured primary cortical neurons imaged with parallelized light sheet fluorescence microscopy.

Congratulations to Kevin Dean for publishing our latest work in Optica (Vol. 4, Issue 2) and contributing the cover image of the current issue. Through parallelization, we report threefold faster imaging speeds in 3D fluorescence microscopy while maintaining the same light dosage and sensitivity of a conventional, slower microscope. This enables long-term, high-speed, and high-resolution volumetric imaging of biological processes that included cell migration, vesicular trafficking, and neuronal action potentials.