Protein Phosphatases and the DNA Damage Response

A main component of the cellular response to DNA damage is a signaling cascade that is driven primarily by phosphorylation events on serine/threonine residues. A significant body of work has identified and characterized the protein kinases and their substrates in this process, but the role of protein phosphatases in the DNA damage response is still in the preliminary stages. Our goal is to identify the phosphatases and the mechanisms regulating these enzymes in the cellular response to DNA damage, with a keen interest on the protein phosphatase 2A (PP2A). We use a combination of biochemistry, cell biology, microscopy, and proteomics to understand how protein phosphatase modulate the DNA damage response.