Representative Publications

Wu, S.-Y., C.F. Lee, H.T. Lai, C.T. Yu, J.E. Lee, H. Zuo, S.Y. Tsai, M.J. Tsai, K. Ge, Y. Wan, and C.-M. Chiang. 2020. Opposing functions of BRD4 isoforms in breast cancer. Molecular Cell 78: 1114-1132.

Bacon, C.W., A. Challa, U. Hyder, A. Shukla, A.N. Borkar, J. Bayo, J. Liu, S.-Y. Wu, C.-M. Chiang, T.G. Kutateladze, and I. D’Orso. 2020. KAP1 is a chromatin reader that couples steps of RNA polymerase II transcription to sustain oncogenic programs. Molecular Cell 78: 1133-1151.

Li, J., Z. Chen, F. Chen, G. Xie, Y. Ling, Y. Peng, Y. Lin, N. Luo, C.-M. Chiang, and H. Wang. 2020. Targeted mRNA demethylation using an engineered dCas13b-ALKBH5 fusion protein. Nucleic Acids Res. 48: 5684-5694.

Lu, L., Z. Chen, X. Lin, L. Tian, Q. Su, P. An, W. Li, Y. Wu, J. Du, H. Shan, C.-M. Chiang*, and H. Wang*. 2020. Inhibition of BRD4 suppresses the malignancy of breast cancer cells via regulation of Snail. Cell Death Differentiation 27: 255-268. (*C.-M. Chiang and H. Wang, co-corresponding authors)

Kim, J.J., S.Y. Lee, F. Gong, A.M. Battenhouse, D.R. Boutz, A. Bashyal, S.T. Refvik, C.-M. Chiang, B. Xhemalce, T.T. Paull, J.S. Brodbelt, E.M. Marcotte, and K.M. Miller. 2019. Systematic bromodomain protein screens identify homologous recombination and R-loop suppression pathways involved in genome integrity. Genes Dev. 33: 1751-1774.

Zhao, J., E.E. Lee, J. Kim, R. Yang, B. Chamseddin, C. Ni, E. Gusho, Y. Xie, C.-M. Chiang, M. Buszczak, X. Zhan, L. Laimins, and R.C. Wang. 2019. Transforming activity of an oncoprotein-encoding circular RNA from human papillomavirus. Nature Communications 10(1): 2300.

Yu, F., G. Shi, S. Cheng, J. Chen, S.-Y. Wu, Z. Wang, N. Xia, Y. Zhai, Z. Wang, Y. Peng, D. Wang, J.X. Du, L. Liao, S.-Z. Duan, T. Shi, J. Cheng, C.-M. Chiang, J. Li, and J. Wong. 2018. SUMO suppresses and MYC amplifies transcription globally by regulating CDK9 sumoylation. Cell Research 28: 670-685.

Iftner, T., J. Haedicke-Jarboui, S.-Y. Wu, and C.-M. Chiang. 2017. Involvement of Brd4 in different steps of the papillomavirus life cycle. Virus Res. 231: 76-82.

Wu, S.-Y., D.S. Nin, A-Y. Lee, S. Simanski, T. Kodadek, and C.-M. Chiang. 2016. BRD4 phosphorylation regulates HPV E2-mediated viral transcription, origin replication, and cellular MMP-9 expression. Cell Reports 16: 1733-1748.

Kim, D.H., S. Kwon, S. Byun, Z. Xiao, S. Park, S.-Y. Wu, C.-M. Chiang, B. Kemper, and J.K. Kemper. 2016. Critical role of RanBP2-mediated SUMOylation of Small Heterodimer Partner in maintaining bile acid homeostasis. Nat. Commun. 7: 12179.

Shu, S., C.Y. Lin, H.H. He, R.M. Witwicki, D.P. Tabassum, J.M. Roberts, M. Janiszewska, S.J. Huh, Y. Liang, J. Ryan, E. Doherty, H. Mohammed, H. Guo, D.G. Stover, M.B. Ekram, G. Peluffo, J. Brown, C. D’Santos, I.E. Krop, D. Dillon, M. McKeown, C. Ott, J. Qi, M. Ni, P.K. Rao, M. Duarte, S.-Y. Wu, C.-M. Chiang, L. Anders, R.A. Young, E.P. Winer, A. Letai, W.T. Barry, J.S. Carroll, H.W. Long, M. Brown, X.S. Liu, C.A. Meyer, J.E. Bradner, and K. Polyak. 2016. Response and resistance to BET bromodomain inhibitors in triple-negative breast cancer. Nature 529: 413-417.

Delcuratolo, M., J. Fertey, M. Schneider, J. Schütz, N. Leiprecht, B. Hudjez, S. Brodbeck, S. Corall, M. Dreer, R.M. Schwab, M. Grimm, S.-Y. Wu, F. Stubenrauch, C.-M. Chiang, and T. Iftner. 2016. Papillomavirus-associated tumor formation critically depends on c-fos expression induced by viral protein E2 and bromodomain protein Brd4. PLoS Pathog. 12: e1005366.

Kalanatari, R., C.-M. Chiang, and D. Corey. 2016. Regulation of mammalian transcription and splicing by nuclear RNAi. Nucleic Acids Res. 44: 524-537.

Chiang, C.-M. 2014. Nonequivalent response to bromodomain-targeting BET inhibitors in oligodendrocyte cell fate decision. Chemistry & Biology 21: 804-806.

Sakakibara, N., D. Chen, M.K. Jang, D. Kang, H. Luecke, S.-Y. Wu, C.-M. Chiang, and A.A. McBride. 2013. Brd4 is displaced from HPV replication factories as they expand and amplify viral DNA. PLoS Pathog. 9: e1003777.

Lai, H.-T. and C.-M. Chiang. 2013. Bimolecular fluorescence complementation (BiFC) assay for direct visualization of protein-protein interaction in vivo. Bio Protocol. 3: e935.

Wu, S.-Y., A-Y. Lee, H.-T. Lai, H. Zhang, and C.-M. Chiang. 2013. Phospho switch triggers Brd4 chromatin binding and activator recruitment for gene-specific targeting. Mol. Cell 49: 843-857.

Chiang, C.-M. 2012. p53-Aurora A mitotic feedback loop regulates cell cycle progression and genomic stability. Cell Cycle 11: 3719-3720.

Wang, W.-M., S.-Y. Wu, A-Y. Lee, and C.-M. Chiang. 2011. Binding site specificity and factor redundancy in activator protein-1-driven human papillomavirus chromatin-dependent transcription. J. Biol. Chem. 286: 40974-40986.

Chiang, C.-M. 2009. Brd4 engagement from chromatin targeting to transcriptional regulation: selective contact with acetylated histone H3 and H4. F1000 Biology Reports 1:98.

Wu, S.-Y. and C.-M. Chiang. 2009. p53 sumoylation: mechanistic insights from reconstitution studies. Epigenetics 4: 445-451.

Wu, S.-Y. and C.-M. Chiang. 2009. Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding. EMBO J. 28: 1246-1259.

Lee, A-Y. and C.-M. Chiang. 2009. Chromatin adaptor Brd4 modulates E2 transcription activity and protein stability. J. Biol. Chem. 284: 2778-2786.

Wu, S.-Y. and C.-M. Chiang. 2007. The double bromodomain-containing chromatin adaptor Brd4 and transcriptional regulation. J. Biol. Chem. 282: 13141-13145.

Wu, S.-Y., A.Y. Lee, S.Y. Hou, J.K. Kemper, H. Erdjument-Bromage, P. Tempst, and C.-M. Chiang. 2006. Brd4 links chromatin targeting to HPV transcriptional silencing. Genes Dev. 20: 2383-2396.

Thomas, M.C. and C.-M. Chiang. 2006. The general transcription machinery and general cofactors. Critical Reviews in Biochemistry and Molecular Biology 41: 105-178.

Thomas, M.C. and C.-M. Chiang. 2005. E6 oncoprotein represses p53-dependent gene activation via inhibition of protein acetylation independently of inducing p53 degradation. Mol. Cell 17: 251-264.

Wu, S.-Y., T. Zhou, and C.-M. Chiang. 2003. Human Mediator enhances activator-facilitated recruitment of RNA polymerase II and promoter recognition by TATA-binding protein (TBP) independently of TBP-associated factors. Mol. Cell. Biol. 23: 6229-6242.

Hou, S.Y., S.-Y. Wu, and C.-M. Chiang. 2002. Transcriptional activity among high and low risk human papillomavirus E2 proteins correlates with E2 DNA binding. J. Biol. Chem. 277: 45619-45629.

Zhou, T. and C.-M. Chiang. 2002. Sp1 and AP2 regulate but do not constitute TATA-less human TAFII55 core promoter activity. Nucleic Acids Res. 30: 4145-4157.

Wu, S.-Y. and C.-M. Chiang. 2002. Expression and purification of epitope-tagged multisubunit protein complexes from mammalian cells. Current Protocols in Molecular Biology, Chapter 16; Unit 16.13

Wu, S.-Y. and C.-M. Chiang. 2001. TATA-binding protein-associated factors enhance the recruitment of RNA polymerase II by transcriptional activators. J. Biol. Chem. 276: 34235-34243.

Zhou, T. and C.-M. Chiang. 2001. The intronless and TATA-less human TAFII55 gene contains a functional initiator and a downstream promoter element. J. Biol. Chem. 276: 25503-25511.

Hou, S.Y., S.-Y. Wu, T. Zhou, M.C. Thomas, and C.-M. Chiang. 2000. Alleviation of human papillomavirus E2-mediated transcriptional repression via formation of a TATA binding protein (or TFIID)-TFIIB-RNA polymerase II-TFIIF preinitiation complex. Mol. Cell. Biol. 20: 113-125.

Wu, S.-Y., M.C. Thomas, S.Y. Hou, V. Likhite, and C.-M. Chiang. 1999. Isolation of mouse TFIID and functional characterization of TBP and TFIID in mediating estrogen receptor and chromatin transcription. J. Biol. Chem. 274: 23480-23490.

Kershnar, E., S.-Y. Wu, and C.-M. Chiang. 1998. Immunoaffinity purification and functional characterization of human transcription factor IIH and RNA polymerase II from clonal cell lines that conditionally express epitope-tagged subunits of the multiprotein complexes. J. Biol. Chem. 273: 34444-34453.

Wu, S.-Y., E. Kershnar, and C.-M. Chiang. 1998. TAFII-independent activation mediated by human TBP in the presence of the positive cofactor PC4. EMBO J. 17: 4478-4490.

Wu, S.-Y. and C.-M. Chiang. 1998. Properties of PC4 and an RNA polymerase II complex in directing activated and basal transcription in vitro. J. Biol. Chem. 273: 12492-12498.

Complete list of publications: