Publications

Publications

2020

Park J.S., Burckhardt C.J, Lazcano R., Solis L.M., Isogai T., Li L., Chen C.S., Gao B., Minna J.D., Bachoo R., DeBerardinis R.J., Danuser G. Mechanical regulation of glycolysis via cytoskeleton architecture. Nature. 2020 Feb;578(7796):621-626. doi: 10.1038/s41586-020-1998-1. 

Jeffrey A. SoRelle, Megan Wachsmann, and Brandi L. Cantarel (2020) Assembling and Validating Bioinformatic Pipelines for Next-Generation Sequencing Clinical Assays. Archives of Pathology & Laboratory Medicine In-Press. Doi: 10.5858/arpa.2019-0476-RA

Jiwoong Kim, David E Greenberg, Reed Pfifer, Shuang Jiang, Guanghua Xiao, Yang Xie, Samuel A Shelburne, Andrew Koh, Xiaowei Zhan. VAMPr: VAriant Mapping and Prediction of antibiotic resistance via explainable features and machine learning. PLoS Comput Biol. 2020 Jan 13;16(1):e1007511. doi: 10.1371/journal.pcbi.1007511. PMCID: PMC7015433 

Zeda Zhang, Chuanli Zhou, Xiaoling Li, Spencer D. Barnes, Su Deng, Elizabeth Hoover, Chi-Chao Chen, Young Sun Lee, Yanxiao Zhang, Choushi Wang, Lauren A. Metang, Chao Wu, Carla Rodriguez Tirado, Nickolas A. Johnson, John Wongvipat, Kristina Navrazhina, Zhen Cao, Danielle Choi, Chun-Hao Huang, Eliot Linton, Xiaoping Chen, Yupu Liang, Christopher E. Mason, Elisa de Stanchina, Wassim Abida, Amaia Lujambio, Sheng Li, Scott W. Lowe, Joshua T. Mendell, Venkat S. Malladi, Charles L. Sawyers, Ping Mu, Loss of CHD1 Promotes Heterogeneous Mechanisms of Resistance to AR-Targeted Therapy via Chromatin Dysregulation, Cancer Cell, 2020. DOI: 10.1016/j.ccell.2020.03.001.

Sruthi Chappidi, Erika C. Villa, Brandi L. Cantarel. Using Mothur to Determine Bacterial Community Composition and Strucutre in 16S Ribosomal RNA Datasets. Current Protocols. 20 June 2019. https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpbi.83

Abdel Nasser Hosein, Huocong Huang, Zhaoning Wang, Kamalpreet Parmar, Wenting Du, Jonathan Huang, Anirban Maitra, Eric Olson, Udit Verma, Rolf A Brekken. Cellular heterogeneity during mouse pancreatic ductal adenocarcinoma progression at single-cell resolution. JCI Insight. 2019;4(16):e129212

Rami R. Hallac, Jeon Lee, Mark Pressler, James R. Seaward, Alex A. Kane. Identifying Ear Abnormality from 2D Photographs Using Convolutional Neural Networks. Scientific Reports. 2019; 9 doi:10.1038/s41598-019-54779-7

Yi-Heng Hao, M. Carmen Lafita-Navarro, Lauren Zacharias, Nofit Borenstein-Auerbach, Min Kim, Spencer Barnes, Jiwoong Kim, Jerry Shay, Ralph J. DeBerardinis & Maralice Conacci-Sorrell. Induction of LEF1 by MYC activates the WNT pathway and maintains cell proliferation. Cell Communication and Signaling. 2019; 17(129). DOI: 10.1186/s12964-019-0444-1


2019

Viren Amin, Didem Ağaç, Spencer D Barnes, Murat Can Çobanoğlu. Accurate differential analysis of transcription factor activity from gene expression. Bioinformatics. btz398, https://doi.org/10.1093/bioinformatics/btz398; PMID: 31099391.

Kopp F, Elguindy MM, Yalvac ME, Zhang H, Chen B, Gillett FA, Lee S, Sivakumar S, Yu H, Xie Y, Mishra P, Sahenk Z, Mendell JT. PUMILIO hyperactivity drives premature aging of Norad-deficient mice. Elife. 2019 Feb 8;8:e42650. doi: 10.7554/eLife.42650. PubMed PMID: 30735131; PubMed Central PMCID: PMC6407921.

He Zhang, Xiaowei Zhan, James Brugarolas, Yang Xie. DEFOR: depth- and frequency-based somatic copy number alteration detector. Bioinformatics. btz170. https://doi.org/10.1093/bioinformatics/btz170; PMID: 30860569.

Ling Cai, ShinYi Lin, Luc Girard, Yunyun Zhou, Lin Yang, Bo Ci, Qinbo Zhou, Danni Luo, Bo Yao, Hao Tang, Jeffrey Allen, Kenneth Huffman, Adi Gazdar, John Heymach, Ignacio Wistuba, Guanghua Xiao, John Minna, Yang Xie. Oncogene (2019) 38:2551–2564. doi: 10.1038/s41388-018-0588-2. PMID: 30532070.

Moore, A. , Wu, L. , Chuang, J. , Sun, X. , Luo, X. , Gopal, P. , Li, L. , Celen, C. , Zimmer, M. and Zhu, H. (2019), Arid1a Loss Drives Nonalcoholic Steatohepatitis in Mice Through Epigenetic Dysregulation of Hepatic Lipogenesis and Fatty Acid Oxidation. Hepatology, 69: 1931-1945. doi:10.1002/hep.30487. PMID: 30584660.

Hashimoto H, Wang Z, Garry GA, Malladi VS, Botten GA, Ye W, Zhou H, Osterwalder M, Dickel DE, Visel A, Liu N, Bassel-Duby R, Olson EN. Cardiac Reprogramming Factors Synergistically Activate Genome-wide Cardiogenic Stage-Specific Enhancers. Cell Stem Cell. 2019 May 6;. doi: 10.1016/j.stem.2019.03.022. PMID: 31080136.

Li S, Chen K, Zhang Y, Barnes SD, Jaichander P, Zheng Y, Hassan M, Malladi VS, Skapek SX, Xu L, Bassel-Duby R, Olson EN, Liu N. Twist2 amplification in rhabdomyosarcoma represses myogenesis and promotes oncogenesis by redirecting MyoD DNA binding. Genes Dev. 2019 Apr 11. doi: 10.1101/gad.324467.119 PMID: 30975722.

Peri-Okonny P, Baskin KK, Iwamoto G, Mitchell JH, Smith SA, Kim HK, Szweda LI, Bassel-Duby R, Fujikawa T, Castorena CM, Richardson J, Shelton JM, Ayers C, Berry JD, Malladi VS, Hu MC, Moe OW, Scherer PE, Vongpatanasin W. High-Phosphate Diet Induces Exercise Intolerance and Impairs Fatty Acid Metabolism in Mice. Circulation. 2019 Mar 12;139(11):1422-1434. doi: 10.1161/CIRCULATIONAHA.118.037550. PMID: 30612451.

Hassounah NB, Malladi VS, Huang Y, Freeman SS, Beauchamp EM, Koyama S, Souders N, Martin S, Dranoff G, Wong KK, Pedamallu CS, Hammerman PS, Akbay EA. Identification and characterization of an alternative cancer-derived PD-L1 splice variant. Cancer Immunol Immunother. 2019 Mar;68(3):407-420. doi: 10.1007/s00262-018-2284-z. PMID: 30564890.

Wang SC, Nassour I, Xiao S, Zhang S, Luo X, Lee J, Li L, Sun X, Nguyen LH, Chuang JC, Peng L, Daigle S, Shen J, Zhu H. SWI/SNF component ARID1A restrains pancreatic neoplasia formation. Gut 2019;68:1259-1270. PMID: 30315093.

Himmelstein DS, Rubinetti V, Slochower DR, Hu D, Malladi VS, et al. (2019) Open collaborative writing with Manubot. PLOS Computational Biology 15(6): e1007128.https://doi.org/10.1371/journal.pcbi.1007128. PMID: 31233491

Min YL, Li H, Rodriguez-Caycedo C, Mireault AA, Huang J, Shelton JM, McAnally JR, Amoasii L, Mammen PPA, Bassel-Duby R, Olson EN. CRISPR-Cas9 corrects Duchenne muscular dystrophy exon 44 deletion mutations in mice and human cells. Sci Adv. 2019 Mar 6;5(3):eaav4324. doi: 10.1126/sciadv.aav4324. PubMed PMID: 30854433; PubMed Central PMCID: PMC6402849.

Stefanius K, Servage K, de Souza Santos M, Gray HF, Toombs JE, Chimalapati S, Kim MS, Malladi VS, Brekken R, Orth K. Human pancreatic cancer cell exosomes, but not human normal cell exosomes, act as an initiator in cell transformation. Elife. 2019 May 28;8:e40226. doi: 10.7554/eLife.40226. PubMed PMID: 31134894; PubMed Central PMCID: PMC6538373.

Miret JJ, Kirschmeier P, Koyama S, Zhu M, Li YY, Naito Y, Wu M, Malladi VS, Huang W, Walker W, Palakurthi S, Dranoff G, Hammerman PS, Pecot CV, Wong KK, Akbay EA. Suppression of Myeloid Cell Arginase Activity leads to Therapeutic Response in a NSCLC Mouse Model by Activating Anti-Tumor Immunity. J Immunother Cancer. 2019 Feb 6;7(1):32. doi: 10.1186/s40425-019-0504-5.  PMID: 30728077.

Amoasii L, Sanchez-Ortiz E, Fujikawa T, Elmquist JK, Bassel-Duby R, Olson EN. Proc Natl Acad Sci U S A. NURR1 activation in skeletal muscle controls systemic energy homeostasis. 2019 Jun 4;116(23):11299-11308. doi: 10.1073/pnas.1902490116. Epub 2019 May 20. PMID: 31110021.

Zhao J, Lee EE, Kim J, Yang R, Chamseddin B, Ni C, Gusho E, Xie Y, Chiang CM, Buszczak M, Zhan X, Laimins L, Wang RC. Transforming activity of an oncoprotein-encoding circular RNA from human papillomavirus. Nat Commun. 2019 May 24;10(1):2300. doi: 10.1038/s41467-019-10246-5. PMID: 31127091. bioRxiv 600056; doi: https://doi.org/10.1101/600056

Abdel Nasser Hosein, Huocong Huang, Zhaoning Wang, Kamalpreet Parmar, Wenting Du, Jonathan Huang, Anirban Maitra, Eric Olson, Udit Verma, Rolf A Brekken. Cellular heterogeneity during mouse pancreatic ductal adenocarcinoma progression at single-cell resolution. JCI Insight. 2019 Jul 23;5. pii: 129212. doi: 10.1172/jci.insight.129212. PMCID: PMC6777805


2018

Henry GH, Malewska A, Joseph DB, Malladi VS, Lee J, Torrealba J, Mauck RJ, Gahan JC, Raj GV, Roehrborn CG, Hon GC, MacConmara MP, Reese JC, Hutchinson RC, Vezina CM, Strand DW. A Cellular Anatomy of the Normal Adult Human Prostate and Prostatic Urethra. Cell Rep. 2018 Dec 18;25(12):3530-3542.e5. doi: 10.1016/j.celrep.2018.11.086. PubMed PMID: 30566875.

Raj K. Gopal, Kirsten Kübler, Sarah E. Calvo, Paz Polak, Dimitri Livitz, Daniel Rosebrock, Peter M. Sadow, Braidie Campbell, Samuel E. Donovan, Salma Amin, Benjamin J. Gigliotti, Zenon Grabarek, Julian M. Hess, Chip Stewart, Lior Z. Braunstein, Peter F. Arndt, Scott Mordecai, Angela R. Shih, Frances Chaves, Tiannan Zhan, Carrie C. Lubitz, Jiwoong Kim, A. John Iafrate, Lori Wirth, Sareh Parangi, Ignaty Leshchiner, Gilbert H. Daniels, Vamsi K. Mootha, Dora Dias-Santagata, Gad Getz, David G. McFadden. Widespread Chromosomal Losses and Mitochondrial DNA Alterations as Genetic Drivers in Hürthle Cell Carcinoma. Cancer Cell. 2018 Aug 13;34(2):242-255.e5. doi: 10.1016/j.ccell.2018.06.013. PMID: 30107175.

Shimada IS, Hwang SH, Somatilaka BN, Wang X, Skowron P, Kim J, Kim M, Shelton JM, Rajaram V, Xuan Z, Taylor MD, Mukhopadhyay S. Basal Suppression of the Sonic Hedgehog Pathway by the G-Protein-Coupled Receptor Gpr161 Restricts Medulloblastoma Pathogenesis. Cell Reports. 2018 Jan 30;22(5):1169-1184. PMID: 29386106.

Wang T, Lu R, Kapur P, Jaiswal BS, Hannan R, Zhang Z, Pedrosa I, Luke JJ, Zhang H, Goldstein LD, Yousuf Q, Gu YF, McKenzie T, Joyce A, Kim MS, Wang X, Luo D, Onabolu O, Stevens C, Xie Z, Chen M, Filatenkov A, Torrealba J, Luo X, Guo W, He J, Stawiski E, Modrusan Z, Durinck S, Seshagiri S, Brugarolas J. An Empirical Approach Leveraging Tumorgrafts to Dissect the Tumor Microenvironment in Renal Cell Carcinoma Identifies Missing Link to Prognostic Inflammatory Factors. Cancer Discovery. 2018 Jun 8. pii: CD-17-1246 (Epub ahead of print). PMID: 29884728.

Pho K, Lu R, Gates S, Xie Y, Lee SJC, Gerber DE. Characteristics of Patients Using Patient Portals in Oncology. JAMA Oncology. 2018 Mar 1;4(3):416-418. PMID: 29372242.

Liu H, Lu R, Shastri S, Sonderman M, Van Buren PN. Assessing Extracellular Volume in Hemodialysis Patients Using Intradialytic Blood Pressure Slopes. Nephron. 2018;139(2):120-130. PMID: 29439257.

Hunter RH, Liu Y, Manjunath H, Acharya A, Jones B, Zhang H, Chen B, Ramanlingam H, Hammer RE, Xie Y, Richardson JA, Rakheja D, Carrol, T, Mendell JT. Loss of Dis3l2 partially phenocopies Perlman syndrome in mice and results in up-regulation of Igf2 in nephron progenitor cells. Genes & Development. 2018 Jul 1;32(13-14):903-908. PMID: 29950491.

Zhang S, Zhou K, Luo X, Tu HC, Sehgal A, Nguyen LH, Zhang Y, Gopal P, Tarlow BD, Siegwart DJ, Zhu H. The Polyploid State Plays a Tumor-Suppressive Role in the Liver. Developmental Cell. 2018 Feb 26;44(4):447-459.e5. PMID: 29429824.

Wang S, Khera R, Das SR, Vigen R, Wang T, Luo X, Lu R, Zhan X, Xiao G, Vongpatanasin W, Xie Y. Usefulness of a Simple Algorithm to Identify Hypertensive Patients Who Benefit from Intensive Blood Pressure Lowering. The American Journal of Cardiology. 2018 Jul 15;122(2):248-254. PMID: 29880288.

MacLean M, Luo X, Wang S, Kernstine K, Gerber DE, Xie Y. Outcomes of neoadjuvant and adjuvant chemotherapy in stage 2 and 3 non-small cell lung cancer: an analysis of the National Cancer Database. Oncotarget. 2018 May 11; 9(36): 24470–24479. PMID: 29849954.

Yang L, Wang S, Gerber DE, Zhou Y, Xu F, Liu J, Liang H, Xiao G, Zhou Q, Gazdar A, Xie Y. Main bronchus location is a predictor for metastasis and prognosis in lung adenocarcinoma: A large cohort analysis. Lung Cancer. 2018 Jun;120:22-26. PMID: 29748011.


2017

Pendleton KE, Chen B, Liu K, Hunter OV, Xie Y, Tu BP, Conrad NK. The U6 snRNA m6A methyltransferase METTL16 regulates SAM synthetase intron retention. Cell. 2017 May 18; 169(5): 824–835.e14. PMID: 28525753.

Zhou H, Morales MG, Hashimoto H, Dickson ME, Song K, Ye W, Kim MS, Niederstrasser H, Wang Z, Chen B, Posner BA, Bassel-Duby R, Olson EN. ZNF281 enhances cardiac reprogramming by modulating cardiac and inflammatory gene expression. Genes & Development. 2017 Sep 1; 31(17): 1770–1783. PMID: 28982760.

Sun X, Wang SC, Wei Y, Luo X, Jia Y, Li L, Gopal P, Zhu M, Nassour I, Chuang HC, Maples T, Celen C, Nguyen LH, Wu L, Fu S, Li W, Hui L, Tian F, Ji Y, Zhang S, Sorouri M, Hwang TH, Letzig L, James L, Wang Z, Yopp AC, Singal AG, Zhu H. Arid1a Has Context-Dependent Oncogenic and Tumor Suppressor Functions in Liver Cancer. Cancer Cell. 2017 Nov 13;32(5):574-589.e6. PMID: 29136504.

Frankel AE, Coughlin LA, Kim J, Froehlich TW, Xie Y, Frenkel EP, Koh AY. Metagenomic Shotgun Sequencing and Unbiased Metabolomic Profiling Identify Specific Human Gut Microbiota and Metabolites Associated with Immune Checkpoint Therapy Efficacy in Melanoma Patients. Neoplasia. 2017 Oct;19(10):848-855. PMID: 28923537.

Spiga L, Winter MG, Furtado de Carvalho T, Zhu W, Hughes ER, Gillis CC, Behrendt CL, Kim J, Chessa D, Andrews-Polymenis HL, Beiting DP, Santos RL, Hooper LV, Winter SE. An Oxidative Central Metabolism Enables Salmonella to Utilize Microbiota-Derived Succinate. Cell Host & Microbe. 2017 Sep 13;22(3):291-301.e6. PMID: 28844888.

Shelburne SA, Kim J, Munita JM, Sahasrabhojane P, Shields RK, Press EG, Li X, Arias CA, Cantarel B, Jiang Y, Kim MS, Aitken SL, Greenberg DE. Whole Genome Sequencing Accurately Identifies Resistance to Extended Spectrum β-Lactams for Major Gram-Negative Bacterial Pathogens. Clinical Infectious Diseases. 2017 Sep 1;65(5):738-745.. PMID: 28472260.

Han T, Goralski M, Gaskill N, Capota E, Kim J, Ting TC, Xie Y, Williams NS, Nijhawan D. Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15. Science. 2017 Apr 28;356(6336). PMID: 28302793.

Jeong S, Kim J, Park W, Jeon H, Kim N. SEXCMD: Development and validation of sex marker sequences for whole-exome/genome and RNA sequencing. PLoS One. 2017 Sep 8;12(9):e0184087. PMID: 28886064.

Ramirez-Martinez A, Cenik BK, Bezprozvannaya S, Chen B, Bassel-Duby R, Liu N, Olson EN. KLHL41 stabilizes skeletal muscle sarcomeres by nonproteolytic ubiquitination. eLIFE. 2017 Aug 9;6. pii: e26439. PMID: 28826497.

Wu H, Bi J, Peng Y, Huo L, Yu X, Yang Z, Zhou Y, Qin L, Xu Y, Liao L, Xie Y, Conneely OM, Jonkers J, Xu J. Nuclear receptor NR4A1 is a tumor suppressor down-regulated in triple-negative breast cancer. Oncotarget. 2017 Aug 15; 8(33): 54364–54377. PMID: 28903348.

Simms-Waldrip TR, Sunkersett G, Coughlin LA, Savani MR, Arana C, Kim J, Kim M, Zhan X, Greenberg DE, Xie Y, Davies SM, Koh AY. Antibiotic-Induced Depletion of Anti-Inflammatory Clostridia is Associated with the Development of GVHD in Pediatric Stem Cell Transplant Patients. Biology of Blood and Marrow Transplantation. 2017 May;23(5):820-829. PMID: 28192251.

Dalvi MP, Wang L, Zhong R, Kollipara RK, Park H, Bayo J, Yenerall P, Zhou Y, Timmons BC, Rodriguez-Canales J, Behrens C, Mino B, Villalobos P, Parra ER, Suraokar M, Pataer A, Swisher SG, Kalhor N, Bhanu NV, Garcia BA, Jeymach JV, Coombes K, Xie Y, Girad L, Gazdar AF, Kittler R, Wistuba II, Minna JD, Martinez ED. Taxane-Platin-Resistant Lung Cancers Co-develop Hypersensitivity to JumonjiC Demethylase Inhibitors. Cell Reports. 2017 May 23;19(8):1669-1684. PMID: 28538184.

Liu N, Garry GA, Li S, Bezprozvannaya S, Sanchez-Oritz E, Chen B, Shelton JM, Jaichander P, Bassel-Duby R, Olson EN. A Twist2-Dependent Progenitor Cell Contributes to Adult Skeletal Muscle. Nature Cell Biology. 2017 March; 19(3): 202–213. PMID: 28218909.

Chuang JC, Budhipramono A, Walker AK, Gevers EF, Ge WP, Eisch AJ, Sun X, Chung AS, Powell CM, Santen GW, Bok LA, Birnbaum SG, Zhu H, Nassour I, Luo X, Nguyen LH, Chahrour M, McEntagart M, Zhang S, Chen F, Celen C, Nijem N, Arid1b haploinsufficient mice reveal neuropsychiatric phenotypes and reversible causes of growth impairment.. eLife. 2017 Jul 11. 6.
PubMed PMID: 28695822. 

Kim J, Xie Y, Williams NS, Ting T.C., Capota E, Nijhawan D, Gaskil N, Goralski M, Han T, Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15.. Science. 2017 Apr 28. 356. 6336. PubMed PMID: 28302793.

Luo X, Zang X, Yang L, Huang J, Liang F, Rodriguez-Canales J, Wistuba II, Gazdar A, Xie Y, Xiao G. Comprehensive Computational Pathological Image Analysis Predicts Lung Cancer Prognosis. Journal of Thoracic Oncology. 2017 March; 12(3): 501–509. PMID: 27826035.

Golden RJ, Chen B, Li T, Braun J, Manjunath H, Chen X, Wu J, Schmid V, Chang TC, Kopp F, Ramirez-Martinez A, Tagliabracci VS, Chen ZJ, Xie Y, Mendell JT. An Argonaute phosphorylation cycle promotes microRNA-mediated silencing. Nature. 2017 February 09; 542(7640): 197–202. PMID: 28114302.

Bhatti MD, Kalia A, Sahasrabhojane P, Kim J, Greenberg DE, Shelburne SA. Identification and Whole Genome Sequencing of the First Case of Kosakonia radicincitans Causing a Human Bloodstream Infection. Frontiers in Microbiology. 2017 Jan 24;8:62. PMID: 28174569.


2016

Zhang YA, Zhou Y, Luo X, Song K, Ma X, Sathe A, Girad L, Xiao G, Gazdar AF. SHOX2 is a Potent Independent Biomarker to Predict Survival of WHO Grade II–III Diffuse Gliomas. eBioMedicine. 2016 Nov; 13: 80–89. PMID: 27840009.

Yousuf Q, Joyce A, Wong T, Frenkel E, Chen W, Kapur P, Josey JA, Wallace EM, Patel N, Ma Y, Zhang H, Homayoun F, Xie Y, Holloman E, Pavia-Jimenez A, Reeves C, Rizzi JP, Geiger H, Christie A, Bruick RK, Kim MS, Gardner KH, McKay RM, Yell P, Sun X, Xie XJ, Hao G, Pedrosa I, Zojwalla N, Courtney K, Hill H, Chang J, Hwang TH, Brugarolas J, Targeting renal
cell carcinoma with a HIF-2 antagonist.. Nature. 2016 Nov 3. 539. 7627. 112-117. PubMed PMID: 27595394.

Kwon WS, Chung HC, Rha SY, Kim SH, Chen KS, Lee WS, Kim HS, Kim J, Heo SJ, Kim HK, Hwang TH, A novel TP53-KPNA3 translocation defines a de novo treatment-resistant clone in osteosarcoma.. Cold Spring Harbor molecular case studies. 2016 Sep. 2. 5. a000992. PubMed PMID: 27626065. 

TeKippe M, Greenberg DE, Raj P, Prokesch BC, Kim J, TeKippe EM, Primary osteomyelitis caused by hypervirulent Klebsiella pneumoniae.. The Lancet. Infectious diseases. 2016 Sep. 16. 9. e190-5. PubMed PMID: 27402393.  

Kim J, Xie Y, Zhan X, Kim MS, Koh AY, FMAP: Functional Mapping and Analysis Pipeline for metagenomics and metatranscriptomics studies.. BMC bioinformatics. 2016 Oct 10. 17. 1. 420.
PubMed PMID: 27724866.