Neuroscience Microscopy Facility
The Neuroscience Microscopy Facility is a research project-oriented core laboratory rather than a fee-for-service facility. We maintain shared imaging and morphology resources for the Department of Neuroscience, including confocal microscope, stereomicroscope, cryostat, ultramicrotome, image data NAS server, and other imaging and morphology-related equipment.
We offer training to new users in the use of the Department's shared-imaging equipment. After a training session, the use of the confocal microscope can be reserved using the log/schedule system.
We also specialize in real-time, long-term gene expression imaging using luciferase reporters in cell/tissue culture. Our group has pioneered the use of firefly luciferase to continuously monitor circadian gene expression in cultured tissues and cells for days to months.
We welcome collaborations, consultation, and training for luminescence imaging for laboratories outside of the Neuroscience Department or UT Southwestern Medical Center.
For collaborations, we provide:
- Consultation for designing and building imaging apparatus
- Consultation for choosing the appropriate reporter for the experimental design
- Training and troubleshooting tissue and cell culture for live cell time-lapse imaging
- Consultation for optimum culture and recording conditions for various reporter genes