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Live Cell Imaging

Our Goal

To enable the application of high-end optical microscopy to cancer-focused research.

Location

C. Kern Wildenthal Research Building (NL)
NL 5 - North Campus
6000 Harry Hines Blvd.
Dallas, TX 75390

Microscopic green image with red lines

The Live Cell Imaging Shared Resource (LCISR) facilitates the application of high-end optical microscopy to the cancer-focused research of Simmons members by providing access to instrumentation, training, and consultation.

Services

LCISR has capability for wide-field, confocal and TIRF imaging of live and fixed samples, including immunofluorescence, fluorescent expression tags, and cleared tissues. The Shared Resource provide instrumentation and expertise that permit multiday, time-lapse imaging of live samples. It provides instrumentation for two photon intra-vital imaging of whole animals and organs, including second harmonic generation for visualizing collagen, muscle, and bone in unstained samples.

LCISR is the primary source for high-quality super-resolution imaging on the UT Southwestern campus and receives significant institutional support as well as support from the Simmons Cancer Center. It provides microscopes capable of true color imaging of histologically stained samples, polarization microscopy, and stereo fluorescence microscopy. It is the only source at UTSW of instrumentation for fluorescence correlation spectroscopy (FCS) in cells and solutions.

Members are provided with access to powerful workstations and software for image processing and analysis. They also work with users to develop customized image acquisition and analysis pipelines, including colocalization, single particle tracking, and object counting.

An additional service made possible by LCISR, in conjunction with the UTSW Electron Microscopy Core Facility, is correlative light and electron microscopy. Also, a new service is a collaboration with UTSW Microscopy Innovation Laboratory (MIL) to fast track access to cutting edge microscope technology for Simmons Cancer Center members.

Technology and Equipment

The LCISR maintains 11 microscopes, two image analysis workstations with software for image processing and analysis, and network-accessible servers for data transfer and temporary backup:

  • Instruments:
    • Zeiss LSM780 Upright scanning confocal/multiphoton
    • Zeiss LSM780 Inverted scanning confocal/multiphoton with live cell incubation
    • Zeiss LSM880 Inverted scanning confocal/multiphoton with live cell incubation and FCS
    • Zeiss LSM880 Inverted scanning confocal with Airyscan with live cell incubation
    • Andor Revolution spinning disk confocal with live cell incubation
    • Two Deltavision deconvolution microscopes with live cell incubation
    • Zeiss Axiovert 200M wide-field microscope with color camera
    • Zeiss Axioscop wide-field microscope with color camera
    • Zeiss SteREO stereomicroscope with fluorescence
    • GE OMX SR super-resolution microscope with 3D SIM, PALM/STORM and Ring TIRF, live cell incubation
    • Dell Precision 7820 workstation, 128 GB RAM, GeForce GTX 1080 GPU card
    • Dell Precision T5500 workstation, 48 GB RAM
    • Autoquant X3, MATLAB, Imaris (floating license) available for image processing and analysis
    • QNAP 32 TB NAS, and two Buffalo Terasation Pro NAS for data storage

Fees

Simmons Cancer Center members and staff receive free training and a 25% subsidy for usage fees.

For additional information, including signing up for services, please visit the LCI Lab Site

Leadership and Contact

Abhijit Bugde, M.S., MBA

Lab Manager, LCSIR

Phone: 214-648-3890

abhijit.bugde@utsouthwestern.edu

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Dorothy Mundy, Ph.D.

Applications Specialist, LCISR

Phone: 214-648-3981

dorothy.mundy@utsouthwestern.edu

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