XF-24(3) Extracellular Flux Analyzer (Seahorse Bioscience)

The XF-24 analyzer simultaneously measures the Oxygen Consumption Rate (OCR) and the Extracellular Acidification Rate (ECAR) of cultured cells, i.e. the instrument allows to measure mitochondrial function in intact cells utilizing real-time bioenergetics - the rate of aerobic respiration and lactic acid production via glycolysis, respectively.

The plate accommodates 24 individual wells, and up to 4 different test compounds (for example, 2-DG, FCCP, 2,4-DNP, Rotenone) can be injected separately into each well.

The instrument detects real-time alterations in cellular metabolism (with a CO2/O2/H ; three Analyte Detection Capability). Furthermore, the system performs label-free, non-destructive measurements, which allow the cultured cells to be utilized for further assays.

Two different prices are available:

$360 per experiment:

• Users will design experiment, create XF assay template, plate cells and analyze data themselves.
• Core facility will supply all XF consumables.
• Core facility will prepare XF cartridge with compounds (oligo > FCCP > Rotenone) for typical bioenergetic profile experiment.
• Core facility will perform media exchange and run assay in XF instrument and send data by email.

$495 per experiment:

• Users will provide cells and offer basic info for experimental design or goal of experiment.
• Core facility will supply all XF consumables.
• Core facility will plate and grow cells for XF assay.
• Core facility will assist with proper experiment design and create XF assay template.
• Core facility will perform media exchange and run assay in XF instrument.
• Core facility will prepare XF cartridge with compounds (oligo > FCCP > Rotenone) for typical bioenergetic profile experiment.
• Core facility will perform data analysis, generation of data summary and send by email.

XF assay takes at least 4 hours. So data will be provided by the next day.

For questions and scheduling, please contact Aktar Ali.