Luminex 100 (cytokines)
The Luminex 100 is a multiplexing machine that uses bead mapping technology to obtain concentrations of multiple cytokines in samples.
Using a patented dying process, Luminex color-codes microscopic beads called microspheres into 100 distinct sets of colors. Then each colored bead set is coated with a reagent specific to a particular bioassay such as antigens, antibodies, oligonucleotides, enzyme substrates, or receptors. When a sample is combined with the microspheres, the reagents on the surface recognize a specific analyte of interest, and capture the analyte through a biochemical binding reaction. Assay results are detected using a flow-cytometry based analyzer, the Luminex 100.
© 2006 Luminex Corporation. Luminex 100 is a trademark of Luminex Corporation.
- What cytokines are available?
The Mouse Metabolic Phenotyping Core offers a large selection of cytokines for human, mouse and rat specimens. Here is a complete list of what we have available:
|
Assays
|
Human
|
Mouse
|
Rat
|
|
IL-1α
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●
|
●
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●
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IL-1β
|
●
|
●
|
●
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IL-1ra
|
●
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IL-2
|
●
|
●
|
●
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IL-2Rα
|
●
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|
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IL-3
|
●
|
●
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IL-4
|
●
|
●
|
●
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IL-5
|
●
|
●
|
●
|
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IL-6
|
●
|
●
|
●
|
|
IL-7
|
●
|
●
|
|
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IL-8
|
●
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|
|
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IL-9
|
●
|
●
|
●
|
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IL-10
|
●
|
●
|
●
|
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IL-12 (p40)
|
●
|
●
|
|
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IL-12 (p70)
|
●
|
●
|
●
|
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IL-13
|
●
|
●
|
●
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IL-15
|
●
|
●
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IL-16
|
●
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|
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IL-17
|
●
|
●
|
●
|
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IL-18*
|
●
|
●
|
●
|
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Basic FGF
|
●
|
●
|
|
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CTACK
|
●
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|
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Eotaxin
|
●
|
●
|
●
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| EGF |
●
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| Fractalkine |
●
|
|
|
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G-CSF
|
●
|
●
|
●
|
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GM-CSF
|
●
|
●
|
●
|
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GRO-α
|
●
|
|
|
| GRO/KC |
|
|
●
|
|
HGF
|
●
|
|
|
|
ICAM-1
|
●
|
|
|
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IFN-α2
|
●
|
|
|
|
IFN-γ
|
●
|
●
|
●
|
|
IP-10
|
●
|
|
●
|
|
KC
|
|
●
|
|
|
LIF
|
●
|
●
|
|
|
MCP-1 (MCAF)
|
●
|
●
|
●
|
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MCP-3
|
●
|
|
|
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M-CSF
|
●
|
●
|
|
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MIF
|
●
|
|
|
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MIG
|
●
|
●
|
|
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MIP-1α
|
●
|
●
|
●
|
|
MIP-1β
|
●
|
●
|
|
|
MIP-2
|
|
●
|
|
|
β-NGF
|
●
|
|
|
|
PDGF-BB
|
●
|
●
|
|
|
RANTES
|
●
|
●
|
●
|
|
SCF
|
●
|
|
|
| sCD40L |
●
|
|
|
|
SCGF-β
|
●
|
|
|
|
SDF-1α
|
●
|
|
|
| TGF-α |
●
|
|
|
|
TNF-α
|
●
|
●
|
●
|
|
TNF-β
|
●
|
|
|
|
TRAIL
|
●
|
|
|
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VCAM-1
|
●
|
|
|
|
VEGF
|
●
|
●
|
●
|
To know what sample volume to provide, please contact the core with a list of cytokines you are interested in testing.
* Not available for single-plexing.
