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| Home > Research > Core Facilities > Mouse Metabolic Phenotyping Core >
Vitros 250, Mouse Metabolic Phenotyping Core
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The Vitros 250

  • Procedure

Collect blood using at least 22-gauge needle to avoid hemolysis.  Serum can be obtained by allowing the blood to clot at room temperature for 30 minutes and then centrifuging at 4000 rpm for 10 minutes, or by using serum separator tubes that can be purchased from Becton Dickenson (order number 365956, vendor: J J Nita Burgoon Co.).  Plasma that has been collected in EDTA tubes cannot be used for most tests.

  • Volume Needed

40ul of dead volume plus 10ul for each analyte measured is required.  Can be plasma or serum.  Samples should be brought to room L5.108 on ice or frozen (note: ALT measurements from frozen samples are not reliable).

  • Tests the machine is capable of running:
Albumin (ALB) Carbon Dioxide (ECO2)
Alcohol (ALC)* Iron (Fe)*          
Alkaline Phosphatase (ALKP) Gamma Glutamyltransferase (GGT)
Alanine Aminotransferase (ALT) Glucose (GLU)
Ammonia (AMON)* Potassium (K)
Amylase (AMYL)* Lactate (LAC)
Aspartate Aminotransferase (AST) Lactate Dehydrogenase (LDH)
Unconjugated/Conjugated Bilirubin (BuBc)* Lipase (LIPA)
Blood Urea Nitrogen (Bun)        Lithium (Li)
Calcium (Ca) Magnesium (Mg)*
Cholinesterase (CHE)* Sodium (Na)
Cholesterol (CHOL)/(HDLC)     Phosphorus (PHOS)
Creatine Kinase (CK) CSF Protein (PROT)*
Creatine Kinase MB (CKMB)* Total Bilirubin (TBIL)
Chloride (Cl)        Total Protein (TP)
Creatinine (CREA) Triglyceride (TRIG)
C-Reactive Protein (CRP) Urine Protein (UPRO)*
Direct HDL (dHDL) Uric Acid (URIC)*

*Please contact the core personnel about these tests BEFORE collecting the blood.

 

  • Prices of each test PER SAMPLE
$1.50        $2.00 $2.50    $5.00  $5.50 $7.00 $7.50 Price available
upon request
ALB    Mg CREA   LIPA   dHDL Li CRP ALC
ALKP   URIC GGT      AMYL   UPRO AMON
ALT     LAC   Fe     CHE
AST                CKMB
BUN              PROT
BuBc              
Ca               
CHOL              
CK              
Cl               
ECO2               
GLU               
             
LDH              
Na               
PHOS              
TBIL              
TP              
TRIG              

 

  • Data and Sample Retrieval

The core will e-mail the results in pdf format to the investigator.  At this time, the investigator will have the option of retrieving any leftover sample.  It will be kept in -4°C for two weeks before being discarded.