Core B: Physiology Core
(NIH Grant number: P30DK079328)
Director: Chou-Long Huang, M.D., Ph.D.
Co-Director: Michel Baum, M.D.
Genetically modified mice are important tools for studying kidney physiology and diseases. However, expertise and equipment for physiological analysis of kidney function in mice are not readily available to many investigators in the renal research community. Furthermore, production of mutant mice has allowed investigators outside the renal community to uncover unexpected renal phenotypes and enter into renal research.
The objective of the Physiology Core is to assist participating (established and new) renal investigators with a wide range of physiological techniques to study kidney physiology and diseases at the cellular, organ, and whole animal levels.
The Core will provide free services for certain assays; will provide advice, equipment and training to assist center investigators’ laboratories to perform other assays; and will arrange collaborations with core directors on certain techniques that require extensive effort and advanced training.
|
Measurement of serum Cr in mice.
- Measurement by capillary electrophoresis provided to participating investigators free of charge.
Contact Person: Dr. Huang (214-648-8627; chou-long.huang@utsouthwestern.edu
Measurement of serum and urine chemistry and blood gas.
- Service provided at costs to investigators. Contact Person: Dr. Baum (214)648-3438;michel.baum@utsouthwestern.edu
Microanalysis of serum and urine electrolytes.
- By flame photometry: service provided free of charge. Contact Person: Dr. Baum (214-648-3438;
michel.baum@utsouthwestern.edu.
- By atomic absorption: Via collaboration only. Contact Person: Dr. Baum (214-648-3438; michel.
baum@utsouthwestern.edu
Blood pressure measurement in mice and rats by tail-cuff method.
- Equipment and training provided to investigators free of charge. Contact Person:
Dr. Baum (214-648-3438; michel.baum@utsouthwestern.edu)
Clearance Studies.
- Core will provide metabolic cages (10 available) and advice to investigators free of charge.
Dr. Huang (214-648-8627; chou-long.huang@utsouthwestern.edu)
Microperfusion; dissection of individual tubules for real time PCR, western blot, and immunohistochemistry.
Updated April 18, 2008 Maintained by Dedrian Copeland
|
