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| Description: |
The Genomics Core is dedicated to the successful use of genomic analysis by Cancer Center members. Our goal is timely, state of the art service, training or advice to members on the use of genomic analysis, with an emphasis on data quality and value to the investigator.
Gene Expression Analysis Services:
Microarray Services: This resource offers the Affymetrix and Illumina platforms for expression analysis. The predominant array layouts are whole genome arrays for both human and mouse, however, other formats are also available such as rat and zebrafish. Each platform offers unique advantages, which can be explained by the Genomics Core Director. The cost varies by array type and vendor.
Quantitative RT PCR: QRTPCR provides an investigator the opportunity to interrogate more modest numbers of genes with higher quantitative accuracy than with array analysis. It is often used to confirm array results. New QRTPCR platforms allow interrogation of expression in 96 or 384 well formats with the potential for multiplexing by using a variety of fluorescence-tagged markers. The core offers QRTPCR analysis in 96-well plate format using either dye intercalation (Syber Green) or tagged primer sets (Taqman).
Genome Analysis Services:
Array-based Comparative Genomic Hybridization (aCGH): aCGH uses the Affymetrix 500K SNP array (250K X 2). Either or both arrays can be used. By analyzing signal intensity at each SNP location DNA copy number can be estimated, thereby providing an investigator with data on the copy number of genes on their samples. This methodology is rapidly replacing BAC arrays for copy number analysis.
Genotyping: The Genomics Core can provide SNP genotyping through the use of the Affymetrix Human Mapping Arrays. These chips come in either 10K, 100K or 500K (250K X 2) formats.
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| Director: |
Michael Story, Ph.D. |
| Contact: |
Kimberley Avila, (214) 648-4992 |
| Location: |
NC7.206 |
Simmons Shared Resources
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