Evaluation of the biochemical and Biophysical Properties of Meibomian lipid Samples collected From Meibomian Glands of normal Donors and Dry Eye Patients

Study ID
STU 122010-158

Cancer Related
No

Healthy Volunteers
Yes

Study Sites

  • UT Southwestern Ambulatory Services

Contact
Mike Molai
214-645-2014
mike.molai@utsouthwestern.edu

Principal Investigator
Igor Butovich

Summary

The first goal of the project is to evaluate the biophysical properties of normal human meibum and correlate those with its biochemical composition in order to establish the ranges within which these parameters change in normals. once this initial goal has been achieved, the next step of the project will be to qualitatively and quantitatively compare normal human meibum with meibum of dry eye patients in order to determine the causes of the poor stability of their tear film, and explore the possibility of using biophysical approaches to diagnose dry eye.

Healthy, non-dry eye subjects and otherwise healthy patients diagnosed with dry eye disease and/or meibomian gland dysfunction including lipogranuloma as a result of an acute or chronic swollen sebaceous gland in the eyelid (chalazia) will be selected to participate in the study. after review and execution of the informed consent, patients will undergo a clinical assessment in order to evaluate whether the subject is non-dry eye or dry eye patient based on the criteria mentioned in the previous section. This visit will be considered a research visit. each consented subject will be assigned a study number and prepared for the biological collection of meibum samples from the ocular lids of both eyes or from the affected gland following [Quote]standard of care[Quote] surgical incision and curettage of the gland. Then, samples will be evaluated in biochemical and biophysical laboratories to determine their composition and biophysical properties. The biochemical analyses will include spectroscopic and chromatographic evaluation of their composition, while their biophysical properties will be determined using primarily the Langmuir trough, Brewster angle microscopy, and calorimetry.

Participant Eligibility

1. Healthy, non-dry eye volunteers and otherwise healthy, dry eye patients (including volunteers with CPB and MGD) of any sex and race, ages 18 and up
2. Patient willing to review, understand, and sign written Informed Consent
3. Written authorization for use or release of health and research study information
4. Dry eye patient must have been diagnosed with dry eye
5. Patient willingness and ability to cooperate with the investigator and follow all instructions