MECHANISMS OF MUSCLE ATROPHY IN SEVERE BURN AND INJURY
First we will test myogenic progenitor cell activity correlated with severity of injury by examining the muscle molecular signals from two groups of injured patients, severe burns and severe trauma. Then we will measure lean mass loss by a number of methods including direct histologic and immunohistochemical analysis and dual-energy x-ray absorptiometry (DeXa), muscle signal transduction expression changes by molecular biology techniques (western-blot and real time q-PCR analysis), and the associated inflammatory response by eLiSa methods. From these data, we will be able to discern changes in myogenic progenitor cell activity in relation to injury severity and inflammatory response. We will then explain the contribution and mechanism of myogenic progenitor cell activity in muscle atrophy after severe injury.
a) >20% TBSA burns or b) ISS>15
Patients with a) <20% TBSA burns or b) ISS<15
At least one planned operation at least 48 hours from injury
Age between 18 and 59