Adhesion Markers for T-Cell Regulatory Activity in Inflammatory Bowel Disease

Study ID
STU 092010-192

Cancer Related
No

Healthy Volunteers
No

Study Sites

Contact
Mary Estess
214-648-4111
pila.estess@utsouthwestern.edu

Principal Investigator
Mark Siegelman

Summary

Patients with inflammatory bowel disease regularly visiting the outpatient Gastroenterology clinic (uT Southwestern ambulatory Services or Parkland Health and Hospital Services) will be asked to donate up to 50 ml of blood at a time. Patients may be drawn on more than one occasion, but not more frequently than once every two months. if possible, blood will be drawn in conjunction with routine blood draws ordinarily performed at clinic visit, although we may request that patients to go to the Parkland outpatient Laboratory or a uT Southwestern ambulatory Services Laboratory to have their blood drawn. isolated blood lymphocytes will be tested for expression of the CD44 adhesion behavior. Results will be compared with the clinical status of the patient at the time of clinic visit as determined by 1)physician assessment and 2)available laboratory criteria, including blood cell counts, blood sedimentation rate, C-reactive protein, auto-antibody profiles, intestinal biopsy results, and other routinely obtained laboratory values.

if and when it has been determined by the patient's physician that intestinal surgery (at university Hospital-St. Paul; university Hospital-Zale; or Parkland Health and Hospital Services) is necessary as a part of the treatment for the patient's inflammatory bowel disease, we will request a portion of that surgically removed tissue. We will only utilize tissue that is medical waste in excess of that which is required for clinical diagnosis or other clinical purposes. The intestinal tissue sample will be used to isolate and characterize particular T lymphocyte subsets that have emigrated into the tissue and may be involved in immune regulation and/or pathogenesis of the autoimmune condition (iBD).

We will isolate and characterize CD4+CD25+ regulatory T cells from peripheral blood and bowel tissue, focusing on those cells expressing the T cell adhesion receptor CD44act. as part of this characterization, we will conduct molecular profiling of T cell receptor (TCR) usage in these cells at the Dna level and characterize the Dna conformation in a particular gene region associated with regulatory T cell function. Please note that a complete genetic profile will not be generated.

Participant Eligibility

Clinically diagnosed with inflammatory bowel disease