Non-Invasive Evaluation of Brain Oxygen Metabolism Using Magnetic Resonance Imaging
The goal of this study is to develop a completely non-invasive method for quantitative measurement of oxygen consumption in the brain. Magnetic resonance imaging (MRI) is a versatile tool for the study of human subjects/patients. It does not require the use of radioactive tracers and is safe for both the patient and the technicians.
The MR signal formation is based on intrinsic substances, typically the proton of the water molecules, which is highly sensitivity to this technique. Furthermore, MRI has a desirable property of high spatial resolution, providing fine details of anatomical structures, and has been widely used in clinical diagnosis. With recent advanced in MRI methodology, it is now possible to use MRI for quantification of many physiological parameters (4).
We and amp;apos;ll be using identical protocol to scan groups of patients with Multiple Sclerosis(MS) and Glucose Transpoter type 1 deficiency(G1D).
MS subjects should be who definite MS clinically, accouding to the revised McDonal criteria and are 18-60 years old.
We propose to study the G1D subjects who have been diagnosed with a very informative type of mutation: a deletion of the entire segment of chromosome 1 where confirmed genotypically via commercially-available DNA sequencing.
The procedures for the liver and kidney oxygen metabolism measurement will be similar to those used in the brain, except that the body of the subject will be aligned to the center of the MRI rather than the brain.
We emphasize again that these procedures are completely non-invasive. We will not use any contrast agent.
The duration of the entire scan session will be approximately 60 minutes. The subject reimbursement plan will also follow that used for the brain MRI scans. Potential risks associated with the liver and kidney studies are expected to be the same as those in the brain studies.
For participants who are enrolled in a new NIH grant, R21 NS078656-01A1, we will also conduct a brief measure of their cognition function. We will focus on the following three domains of cognitive functions. These measurements will be made by Dr. Denise Park[Right Quote]s group at UTD.
PROCESSING SPEED. This measure assesses the rate at which information is processed and predicts a large amount of age-related variance in cognitive function. Test used will be Digit Comparison.
WORKING MEMORY. This construct provides a measure of on-line processing capacity, integrating both storage and processing capacity, and is highly related to memory function and reasoning. Instrument used will be WMS Letter – Number Sequencing.
LONG TERM MEMORY FUNCTION. The tasks below measure long-term memory function, which involves both frontal and hippocampal components. Task used will be Woodcock Johnson Test.
There is no known risk associated with their cognitive measurements, although they may feel bored
The inclusion criteria ages will be 18-79 years old.
For the EEG (Electroencephalogram) session, we will again use protocol that has been applied extensively in healthy subjects (see page 4). Briefly, the volunteer will undergo an EEG recording of their brain wave activity while performing the gas inhalation task.
• Mentally competent to give informed consent
• Males and females
• Ages 18-79 years old
• All races and ethnicities
• Able to read, speak, and understand English
For G1D study, we'll recruit thirty healty subjects and thirty G1D patients who are ten years of age and older.