Proteomic Changes in the Aging Tear Film
This is a single center clinical study to analyze the tear film proteome in normal tears and assess changes that may occur in response to aging. To allow for a robust analysis with a relatively small sample size, a generational study will be performed evaluating female members of a single family (grandmother, daughters, and granddaughters).Healthy, non-fasted volunteers without preexisting ocular disease will be recruited a total of approximately 12 subjects will be recruited for this study from a single family from at least 3 generations. Minors will be eligible and included in this study in order to find a single family and include 3 generations. There is a strong possibility that due to the ages of the family members from the generations that a minor will be in the younger generation. This population can also contribute to the overall nature of this study in comparing the proteomic make up of their tears compared to the older generations. Dry eye testing, consisting of a brief slit lamp exam and routine tear testing (i.e.: Schirmer's test for aqueous tear production, tear break up time for tear film stability, fluorescein and lissamine green staining, and measurement of the inferior tear meniscus height) will be performed to evaluate for dry eye status. Dry eye status will only be assessed at the first clinical visit. Five microliters of basal tear fluid will be collected from the lateral canthus using micro-capillary tubes and stored at -80[Degrees]C, until use. Repeat sample collections will be performed on three additional days for a total of 4 study visits. Tear collection will be performed between 8am-12 pm for each visit. . .. Samples from both eyes at all visits will be pooled for each individual patient (for a total of 40ul of tear fluid from each subject). Samples will be subject to SDS-PaGe, stained with coomassie blue, and sent to our campus proteomics facility for analysis.
1. Healthy, non-smoking females without any ocular or systemic complications, with the exception of hypertension and high cholesterol
2. Non-contact lens wearers (or no past history of contact lens wear for prior30 days)for females of any race or origin, ages 18 and up
3. Patients that are willing to review, understand, and sign the written Informed Consent
4. Written authorization for use or release of health and research study information
5. Patients that are willing and able to cooperate with the Investigator and follow all instructions