Defining the Tear Film Proteome
This is a single center clinical study to analyze the tear film proteome in normal tears .To allow for a robust analysis with a relatively small sample size, this study will be performed evaluating monozygotic and dizygotic twins. Healthy, non-fasted volunteers without preexisting ocular disease will be recruited. a total of approximately 20 subjects will be recruited for this study (5 sets of each type of twin) Dry eye testing, consisting of a brief slit lamp exam and routine tear testing (i.e.: Schirmer's test for aqueous tear production, tear break up time for tear film stability, fluorescein and lissamine green staining, and measurement of the inferior tear meniscus height) will be performed to evaluate for dry eye status. Dry eye status will only be assessed at the first clinical visit. Ten microliters of basal tear fluid will be collected from the lateral canthus using micro-capillary tubes and stored at -80[Degrees]C, until use. Repeat sample collections will be performed on three additional days for a total of 4 study visits. Tear collection will be performed between 7am-12 pm for each visit. Samples from both eyes at all visits will be pooled for each individual patient (for a total of 80ul of tear fluid from each subject). Samples will be subject to SDS-PaGe, stained with coomassie blue, and sent to our campus proteomics facility for analysis.
1. Healthy, non-smoking females without any ocular or systemic complications, with the exception of hypertension and high cholesterol
2. Non-contact lens wearers (or no past history of contact lens wear for prior30 days)for females of any race or origin, ages 18 and up
3. Patients that are willing to review, understand, and sign the written Informed Consent
4. Written authorization for use or release of health and research study information
5. Patients that are willing and able to cooperate with the Investigator and follow all instructions