Establishment of Urologic Tissue and Serum Repository
The proposed project deals with a campus-wide organization of harvesting and storage of tissue, blood and body fluid obtained from well patients and those suffering from a variety of urologic diseases. Although the vast majority of specimens will be malignant tissues removed because of urologic cancer, we also plan to obtain samples from well patients and those with benign neoplasms and conditions (e.g. BPH, neuropathic bladder disease, and nephrolithiasis). All tissue specimens obtained whether as the result of surgery for benign or malignant disease will be examined and processed by the surgical pathologist involved in the proposed project. The majority of the tissue will be utilized for routine histology within the Department of Surgical Pathology as part of routine care; for example, in order to determine the pathologic stage, margin status and grade of the tumor in a case of cancer. Selected portions that are deemed not essential to these goals will then be obtained and stored within the urologic tissue repository for future analysis. Where possible under the discretion of the surgical pathologist, normal adjacent tissue from the diseased organ may also be obtained for control purposes. At the time of surgery, we also propose to obtain urine and 20 cc of blood from each patient from whom tissue is collected as well as from well patients from whom no tissue is collected. This will provide researchers not only with diseased tissue specimens, but also urine and blood or other body flluids from which control normal DNA may be obtained. Stored urine and blood or other body fluids also may allow research into inadequately researched factors which may be clinically useful markers of disease presence or prognosis. Informed consent will be obtained for the procurement of all specimens.
The majority of the patients will be undergoing surgery for urologic cancer. Nonetheless, we will also obtain blood, urine or other body fluids or tumor specimens from well patients as well as those with other urologic malignancies and benign conditions which may occur in younger individuals. Patients of all ethnic backgrounds will be included and it is anticipated that some patients will be in poor general health status due to the nature of their diseases (for example, advanced cancer). No patient, however, will undergo tissue removal for the sole purposes of retrieving tissue for the repository. Rather, if surgical or medical indicators require the removal of tissue specimens for therapeutic or diagnostic purposes, efforts will be made to include portions not needed for routine care in the tissue repository. Patients will not suffer harm or addition surgical intervention beyond the risk inherent in the medically indicated therapeutic or diagnostic procedures.