Evaluation of the biochemical and Biophysical Properties of Meibomian lipid Samples collected From Meibomian Glands of normal Donors and Dry Eye Patients

Study ID
STU 122010-158

Cancer Related
No

Healthy Volunteers
No

Study Sites

  • UT Southwestern Ambulatory Services

Contact
Yesenia Leach
214/645-3087
Yesenia.Leach@UTSouthwestern.edu

Principal Investigator
Igor Butovich, Ph.D.

Summary

The main goals of the project are: (1) To evaluate the biophysical properties of normal human meibum and establish a normal biochemical composition range within which these parameters change. and to qualitatively and quantitatively compare normal human meibum with meibum of dry eye patients in order to determine the causes of the poor stability of their tear film, and explore the possibility of using biophysical approaches to diagnose dry eye;(2) to compare the biochemical composition of meibum, sebum and tear lipids to elucidate the origin of the tear lipidome; (3) to determine whether the molecular composition and quality of meibum can be affected by systemic lipid metabolism disorders and/or pharmaceutical interventions.

Participant Eligibility

Normal group:
1. Healthy, non-dry eye volunteers of any sex and race, ages 18 and up to 80 years old
2. Patient willing to review, understand, and sign written Informed Consent
3. Written authorization for use or release of health and research study information
4. Patient willingness and ability to cooperate with the investigator and follow all instructions

Dry Eye Group:
1. Dry eye patients (including volunteers with CPB and MGD) of any sex and race, ages 18 and up, previously diagnosed
2. Patient willing to review, understand, and sign written Informed Consent
3. Written authorization for use or release of health and research study information
4. Patient willingness and ability to cooperate with the investigator and follow all instructions

Lipid Metabolism disorders Group:
1. Must meet one or more of the following findings in blood lipid levels:

* LDL-C >= 120 md/dl

* TG = 160 - 500 mg/dl

* Non HDL-C ≤ 160 mg/dl