Protein Chemistry Core - Peptide Synthesis
We have two multiple peptide synthesizers that are able to synthesize up to 48 peptides simultaneously at either 25, 50 or 100 µmol scales. In addition, our Perseptive Biosystems Pioneer and Applied Biosystems 433 synthesizers are able to synthesize 1 or 2 peptides at either a 0.1 or 0.25 mmol scale and is the preferred instrument for the stepwise synthesis of peptides longer than 20 residues, using Fmoc chemistry. Our most recent acquisition is an Applied Biosystems 430 synthesizer that has been optimized for the synthesis of polypeptides up to 100 residues, using t-Boc chemistry. We are also equipped for chemically synthesizing small proteins using native chemical ligation technology.
Turnaround time is usually 7 days for crude peptides and 7-10 days for purified peptides. All peptides are delivered with a mass spec analysis and a reversed-phase HPLC chromatogram to confirm purity and identity.
Crude peptides are delivered as a dried powder and percentage purity is usually dependent on the sequence being synthesized. Minimum purity for crude material will be 70%. Purified peptides will be at least 95% pure, based on the accompanying reversed-phase HPLC profile. The pure material will be characterized by a mass spectrum and delivered as a lyophilized powder.
|5-50 mg||1-10 mg|
|50||10-100 mg||2-20 mg|
|100||20-200 mg||4-40 mg|
|250||50-500 mg||10-100 mg|