Protein Chemistry Core - Equipment Roster

Major Equipment

Peptide Synthesis

Applied Biosystems 433A: Single peptide, batch synthesizer with Fmoc and t-Boc chemistry capability at a scale of 0.1 to 0.5 mmol.

Applied Biosystems 430A: Single peptide, batch synthesizer with Fmoc and t-Boc chemistry capability at a scale of 0.1 to 0.5 mmol. Fast cycles allow a 20mer peptide to be synthesized in 8h.

Perseptive Biosystems Pioneer: Fmoc chemistry only, continuous flow instrument, which will synthesize 2 peptides simultaneously.

Rainin Symphony: Multiple peptide synthesizer using batch synthesis methodology and either Fmoc or t-Boc chemistry. Up to 12 peptides can be synthesized simultaneously.

Advanced Chemtech APEX396: Multiple peptide synthesizer using batch synthesis methodology and either Fmoc or t-Boc chemistry. Number of peptides that can be synthesized at one time depend on the RV block used and the desired synthesis scale.

Peptide Institute Hydrogen Fluoride Apparatus: Permits the safe handling of HF required for final cleavage and deprotection of t-Boc synthesized peptides.

Mass Spectrometry

Waters LR Reflectron MALDI-TOF: High sensitivity, high resolution MALDI-TOF with reflectron capability.

Protein Sequencing

Applied Biosystems 494: We have two 4-channel Edman protein sequencers, one of which is dedicated for handling P³²-labelled samples.