Kidney Core Request Form

Generation and distribution of transgenic mice expressing kidney specific tetracycline-inducible activator and repressor (Ksp/rtTA and KsptTA).
Assistance in the generation of additional transgenic mice and knockout and knockin mice.
Training investigators to perform embryonic kidney organ cultures.
Distribution of transgenic Mice--Ksp/Cre (Please Specify Gene & Brief Research Plan in Special Instructions)
The Ksp/Cre Mice are now available through Jackson Laboratories
Distribution of transgenic Mice---Ksp.CreERT2 (Please Specify Gene & Brief Research Plan in Special Instructions)
Distribution of transgenic Mice---Pkhd1/Cre (Please Specify Gene & Brief Research Plan in Special Instructions)
Performing and training investigators in the use of kidney injury/repair models including renal ischemia-reperfusion injury, ureteral obstruction, chemical injury and bone marrow stem cell transplantation
Distribution of Kif3a mutant (and wildtype)cell lines

NOTE: We will begin charging $100/mouse for requests received after July 31, 2012. As always, shipping will be paid by the recipient.

Number of Samples
Measurement of urine and serum Cr in mice by capillary electrophoresis.
Measurement of serum and urine chemistry and blood gas.
Microanalysis of serum and urine electrolytes.
Blood pressure measurement in mice and rats by tail-cuff method. Equipment and training provided to investigators free of charge.
Clearance Studies. Core will provide metabolic cages (10 available) and advice to investigators free of charge.
Microdissection of tubules for quantitation of proteins/gene expression (By collaboration only.)
Microperfusion studies of renal tubules (By collaboration only).
Electrophysiological Studies of ion Channels by patch-clamp recordings (By collaboration only).

Physiology Core Special Instructions.

NOTE: For requests submitted after July 31, 2012, there will be a $3/assay charge for creatinine assays and a $15/sample charge for chemistry panels and blood gas.

Click here for sample submission guidelines.

Histology (tissue fixation, embedding, and staining).
Consult for immunohistochemistry/lectin and enzyme histochemistry and in situ hybridization.
Light and confocal microscopy and image analysis.
Laser microdissection.
Kidney pathology
Training in select procedures.
Distribution of plasmids.

Cell Biology & Pathology Special Instructions

Please indicate which of the following categories your study falls under. NOTE: As of August 1, 2012, we will begin charging for some services as indicated below.

Investigator initiated study           Industry sponsored study

Service(s) Requested:

Human research protocol design, methods, and implementation and conduct.
Study participant recruitment and management.

Regulatory document preparation and monitoring. $20/hour
Initial Amendment Renewal

Biostatistical support for both human and laboratory studies.
Biological sample collection (blood, urine, nail clippings, hair, etc.), preparation, storage and transportation. $20/hourInternal Analysis? yes no
If yes – please provide sample type: Data collection for phenotyping human research subjects. $20/hour
Database development and management.

Patient Management

ABPM ($100/test)   FMD ($300/test)   PWV ($100/test)
Central Aortic Pressure ($100/test)

Specialized analyses of serum and urine analytes including iothalamate.
$7.00/iothalamate assay (click here for iothalamate analysis sample guidelines);
$3.00/creatinine assays (click here for creatinine analysis sample guidelines)
Training in methods of clinical and translational research including protocol development, human research subject, and institutional review board documentation. $20/hour