Cell Biology & Pathology Core
The objective of this core is to provide high quality histopathologic and scientific support for the research projects by localizing proteins and/or mRNAs in tissues and cultured cells and to offer pathology support. The core maintains a library of wild-type murine embryos and adult tissues embedded in paraffin for use by O'Brien Center investigators. The lab also maintains bright-field, dark-field, phase-contrast, epifluorescence, and confocal microscopes. Orson Moe, MD, and James Richardson, DVM, PhD, have an established history of expertise and competence in a wide range of histological and imaging techniques.
The most frequently used services include:
- Processing of tissues for routine paraffin embedding
- Performing detailed paraffin and cryo-microtomy
- Performing standard histologic stains and special stains when required
- Performing immunohistochemistry and in situ hybridization
- Immunocytochemistry and single tubule immunohistochemistry
- Light and confocal microscopy and image analysis
- Kidney pathology
- Training in the above procedures
Mode of Operation
- Investigators and their key personnel meet with Core Directors and staff before a project is launched.
- The Core Directors are actively involved in the experimental design of the various protocols.
- The investigators determine the level of involvement from their laboratory. The Core may provide all the service or the investigator can work collaboratively with the Core.
- A mutually agreed upon plan is formulated, and the estimated cost recovery and time line is presented to the investigator.