The study of genetically modified mice has greatly expanded our understanding of kidney development and pathophysiology. The UT Southwestern O’Brien Center Animal Models Core will provide cutting edge technology to investigators wishing to generate mouse models for the study of kidney development and/or function.
The core will serve three major purposes. One is to provide the necessary reagents and expertise to mouse geneticists who do not normally study the kidney to facilitate and encourage their move into this field. The second is to encourage non-mouse geneticists, especially clinical investigators, to venture into the mouse system and to ease this transition. The third is to provide tools to mouse geneticists who do study the kidney to assist in their studies. This will be accomplished in several ways.
First, the core will generate and distribute several kidney-specific Cre recombinase and tetracycline-inducible activator and repressor lines to be crossed to co-investigator-provided floxed or tet responder lines.
Second, core personnel will assist in the generation of additional transgenic mice, knockout/in mice and cell lines as required by co-investigators.
Finally, core personnel will perform and train investigators in the use of various models of organ injury/repair and ex vivo culture.
As mentioned, the ultimate purpose of this core is to facilitate research on kidney development and pathophysiology.
- Generation and distribution of transgenic mice expressing kidney specific tetracycline-inducible activator and repressor (Ksp/rtTA and KsptTA).
- Assistance in the generation of additional transgenic mice and knockout and knockin mice
- Training investigators to perform embryonic kidney organ cultures
- Distribution of transgenic Mice — Ksp/Cre (Please Specify Gene & Brief Research Plan in Special Instructions) (The Ksp/Cre Mice are now available through Jackson Laboratories)
- Distribution of transgenic Mice — Ksp.CreERT2 (Please Specify Gene & Brief Research Plan in Special Instructions)
- Distribution of transgenic Mice — Pkhd1/Cre (Please Specify Gene & Brief Research Plan in Special Instructions)
- Performing and training investigators in the use of kidney injury/repair models including renal ischemia-reperfusion injury, ureteral obstruction, chemical injury and bone marrow stem cell transplantation
- Distribution of Kif3a mutant (and wildtype) cell lines
NOTE: We will begin charging $100/mouse for requests received after July 31, 2012. As always, shipping will be paid by the recipient.