Equipment

Imaging Systems

Zeiss LSM510 Confocal with META

Confocal Microscope
Zeiss LSM510 Confocal

Uses: Optical sectioning of immunofluorescent staining, colocalization analysis, quantitative measurements (cell diameter, spine length), and works well with Volocity and MicroBrightField Systems

Microscope: Zeiss Axiovert 200

Filters: CY2, CY3, DAPI

Objectives: 5X, 0.16 NA; 10X, 0.3 NA; 20X, 0.75 NA; 63X, 1.2 (Water); 100X, 1.4 NA (Oil)

Laser lines: Argon (477,488,514), HeNe1 (543), HeNe2 (633)

Notes: The LSM510 confocal is co-sponsored by the Department of Psychiatry and the Simmons Cancer Center. The confocal features META. Priority is given to permanent members of the Department of Psychiatry and the Simmons Cancer Center. All users must be trained/approved prior to use. All users pay an hourly fee.

Training: Small group, quarterly

Calendar: Online at Teamup.com

MicroBrightField (MBF) System

Microbrightfield System
MicroBrightField System

Uses: Stereology (the unbiased estimation of the number, length, area, and volume of cells/structures; Stereo Investigator Software) and Dendrite/Spine Analysis (Neurolucida Software)

Microscope: Olympus BX51

Camera: Optronics Microfire color camera

Filters: CY2, CY3, CY5, DAPI

Objectives: 2X, 0.05 NA; 4X, 0.16 NA; 10X, 0.3 NA; 20X, 0.5 NA; 40X, 0.75 NA; 100X, 1.3 NA (Oil)

Software: MicroBrightField licensed software, both Stereo Investigator (version 11.03) and Neurolucida (version 10)

Notes: Priority is given to permanent members of the Department of Psychiatry. All users must be trained/approved prior to use. All users pay an hourly fee.

Training: Individual, as needed

Calendar: Online at Teamup.com

Right Epi/BrightField Microscope

Microscope
Olympus BX60

Uses: Brightfield and fluorescence microscopy, image acquisition, and teaching/training

Microscope: Olympus BX60

Camera: Olympus DP70

Filters: FITC, CY3, DAPI

Objectives: 4X, 0.16 NA; 10X, 0.3 NA; 20X, 0.5 NA; 40X, 0.95 NA; 60X, 1.4 NA (Oil); 100X, 1.3 NA (Oil)

Training: Individual, As Needed

CalendarOnline at Teamup.com

Left Epi/BrightField Microscope

Microscope
Olympus BX51

Uses: Brightfield and fluorescence microscopy, image acquisition, and teaching/training

Microscope: Olympus BX51

Camera: Olympus DP70

Filters: FITC/GFP, EYFP, CY3, CY5, DAPI

Objectives: 2X, 0.08 NA; 4X, 0.13 NA; 10X, 0.4 NA; 20X, 0.7 NA; 40X, 0.75 NA; 40X, 1.35 NA (Oil); 100X, 1.4 NA (Oil)

Training: Individual, As Needed

CalendarOnline at Teamup.com

Tissue Culture Microscope

Microscope
Nikon Inverted Microscope

Uses: For tissue culture room use only, assess confluence/viability/infection/etc. of cells, fluorescence and brightfield microscopy, and image acquisition

Microscope: Nikon Inverted Microscope

Camera: Leica DFC450C Cooled Color 5MP Camera

Objectives: 2X, 0.05 NA; 4X, 0.13 NA; 10X, 0.3 NA; 20X, 0.75 NA; 40X, 0.55 NA; 40X, 0.85 NA

Filters: DAPI, CY2, CY3

Training: Individual, As Needed

Dissecting Microscope

Microscope
Olympus Stereomicroscope

Uses: For tissue culture room use only, brightfield microscopy, tissue dissections, teaching/training

Microscope: Olympus Stereomicroscope

Magnification: 0.67X to 4.5X

Accessories: Specimen illumination lamp, camera attached to 32" LCD TV

Training: Individual, As Needed

Macroscope

Macroscope
Olympus Stereomicroscope

Uses: For gross tissue observation (cannula placement, ventricle size, etc.), tissue dissections, brightfield microscopy only, image acquisition

Microscope: Olympus Stereomicroscope

Magnification: 0.67X to 4X

Camera: Nikon Coolpix 4500

Training: Individual, As Needed