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Fixation Methods & Recipes

To assist client investigators, the Molecular Pathology Core provides fixation recipes and procedures for quick reference. The hosted files suggest methods for best quality routine histology as well as methods for preserving antigens and chemical moieties sometimes lost through routine procedures. The listed documents are in MS Word xml format (.docx) and readily downloadable.

Fixation Methods
Organ Fixation for Paraffin and Frozen Histology
Transcardial Perfusion

Recipes
Krebs-Henseleit Cardiac Relaxant
4% Paraformaldehyde General Fixative
Methyl Carnoys Mucin Fixative
Periodic Acid Glycogen Fixative

Other
Primer on Collaborative ISH