I am studying O-GlcNAc protein's interactions by combining chemistry and biology technology.
Many proteins in metazoa have O-GlcNAc modification as one of important post-translational modifications. But the functional roles of this modification are still remain elusive at the molecular level. Recently, I have developed a metabolic engineering method that can label cellular O-GlcNAc proteins with a photocrosslinkable group, which can make covalent conjugation between O-GlcNAc proteins and their interacting partner upon UV irradiation in live cell. I believe this new tools will be useful to elucidate many of important O-GlcNAc mediated protein's functional regulations.
Yu, S-H, Wands, AM & Kohler, JJ. Photoaffinity probes for studying carbohydrate biology. Journal of Carbohydrate Chemistry (2012) 31: 325-352.
Yu, S-H, Boyce, M, Wands, AM, Bond, MR, Bertozzi, CR, & Kohler, JJ.Metabolic labeling enables selective photocrosslinking of O-GlcNAc-modified proteins to their binding partners, Proc. Natl. Acad. Sci. USA, (2012) 109: 4834-4839.
M. Boyce, I. S. Carrico, A. S. Ganguli, S. H. Yu, M. J. Hangauer, S. C. Hubbard, J. J. Kohler, C. R. Bertozzi, “Metabolic crosstalk allows labeling of O-linked β-N-acetylglucosamine-modified proteins via the N-acetylgalactosamine salvage pathway”, PNAS, 2011, 108, 3141-3146.
J. E. Rexach, C. J. Rogers, L. C. S. H. Yu, J. Tao, Y.E. Sun, L. C. Hsieh-Wilson, “Quantification of O-GlcNAc Glycosylation Stoichiometry and Dynamics using Resolvable Mass Tags”, Nat. Chem. Biol. 2010, 6, 645-651.
S. H. Yu, M. R. Bond, C. M. Whitman, J. J. Kohler, “Metabolic labeling of glycoconjugates with photocrosslinking sugars” Methods in Enzymology, 2010, 478, 541-561.
K. C. Seo, S. H. Yu, S. K. Chung, " Divergent Synthesis of all possible optically active Regioisomers of Myo-inositol mono- and bisphosphates” J. Carbohydr. Chem. 2007, 26, 305-327.
J.J. Park, S. H. Yu, S. K. Chung, “Construction of SphingolipidsLibraries and their Utilities” Int. J. Soc. Mater. Eng. Resour. 2006, 14, 18-21.
S. H. Yu, J. J. Park, and S. K. Chung, Practical syntheses of optically active carbagalactose and their potential application to the carbocyclic analogues of KRN7000” Tetrahedron: Asymmetry 2006, 17, 3030-3036.
S. H. Yu and S. K. Chung, "Divergent syntheses of all 16 carbasugar stereoisomers via stereoconversion of carba-β-D-altropyranose derivatives” Tetrahedron: Asymmetry 2005, 16, 2729-2747.
N. Y. Lee, W. J. Jang, S. H. Yu, Jungkyun Im and Sung-Kee Chung. "Syntheses of glycodendrimers having scyllo-inositol as the scaffold", Tetrahedron Lett. 2005, 46, 6063-6066.
S. H. Yu and S. K. Chung, "Practical syntheses of enantiopure carbasugars: carba-β-altrose, carba-β-mannose, carba-β-idose, and carba-β-talose derivatives", Tetrahedron: Asymmetry 2004, 15, 581-584.
S. K. Chung, S. H. Yu, and Y. T. Chang, "Syntheses of D-myo-Inositol 1,2,6-trisphosphate and 2,6-bisphosphate, J. Carbohydr. Chem. 1998, 17, 385-390.
S. K. Chung and S. H. Yu, " Ferrier Reaction of Methyl α-D-Mannopyranoside: Synthesis of L-chiro-inositol 1,2,3-trisphosphate and L-chiro-inositol 1,2,3,5-tetrakisphosphate ", Kor. J. Med. Chem. 1996, 6, 35-46.
S. K. Chung and S. H. Yu, "Synthesis of L-chiro-inositol 1,2,3-trisphosphate and L-chiro-inositol 1,2,3,5-tetrakisphosphate by Ferrier Reaction of Methyl α-D-Mannopyranoside", Bioorg. Med. Chem. Lett. 1996, 6, 1461-1464.
S. K. Chung and S. H. Yu, " Synthesis of D-6-deoxy-6,6-difluoro-myo-inositol ", Kor. J. Med. Chem. 1994, 4, 80-83.