Research

In vitro analysis of cortical actin-membrane microcluster interactions

 

The actin cortex adjacent to the plasma membrane seems to play a dual role in regulating the organization and dynamics of membrane-associated proteins, both confining them and aiding their mobility.

In order to shed light on this dual function, we are collaborating with the Rosen lab at UTSW to study an in vitro system that reconstitutes membrane protein clustering in an artificial lipid bilayer with an adjacent acto-myosin meshwork. The molecular manipulation power of in vitro systems combined with quantitative analysis will allow us to thoroughly characterize the molecular mechanisms underlying the regulation of microcluster growth and dynamics in the membrane by the actin meshwork.