Vector Core

Adenoviral Shuttles

NameMap
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Sequence
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Description
pACtreCMV Map Sequence Adenoviral shuttle plasmid with tet-regulated promoter and SV40 polyA site for cDNA cloning. Shuttle used to produce Ad5 E1 replacement vectors with the E3 region containing a tet-ON transgene using homologous recombination in permissive cells.
pACU6loxP Map Sequence Adenoviral shuttle plasmid with U6 pol3 promoter, SalI and XbaI sites for precise cloning of synthetic oligos encoding shRNAs, stem/terminator and loxP sites. Shuttle used to produce Ad5 E1 replacement vectors using cre/lox recombination.
pAdCMVpL(-)iresGFPloxP Map Sequence Adenoviral shuttle plasmid with CMV immediate early promoter, partial pUC19 polylinker, iresGFP, SV40 splice/polyA, and restriction tag SwaI, SfiI, PmeI, & NheI for linearization. Shuttle used to produce Ad5 E1 replacement vectors that co-express eGFP using cre/lox recombination following cDNA cloning.
pAdCMVpLpA(-)loxP Map Sequence Adenoviral shuttle plasmid with CMV immediate early promoter, pUC19 polylinker, SV40 splice/polyA, loxP and restriction tag SwaI, SfiI, PmeI, & NheI for linearization. Shuttle used to produce Ad5 E1 replacement vectors using cre/lox recombination following cDNA cloning.
pAdpL(+)loxP Map Sequence Adenoviral shuttle plasmid with unique polylinker for gene insertion and restriction tag SwaI, SfiI, PmeI, & NheI for linearization. Does not contain a promoter or splice/polyA site. Shuttle used to produce Ad5 E1 replacement vectors using cre/lox recombination.
pAdRSVpLpA(-)loxP Map Sequence Adenoviral shuttle plasmid with RSV promoter, pUC19 polylinker, SV40 splice/polyA, loxP and restriction tag SwaI, SfiI, PmeI, & NheI for linearization. Shuttle used to produce Ad5 E1 replacement vectors using cre/lox recombination following cDNA cloning.