Preparing and using recombinant viral vectors requires specialized and expensive techniques, reagents, and biosafety-compliant laboratory space not available to every investigator.
The Vector Core Laboratory provides the scientific resources necessary to produce state-of-the-art recombinant adenoviruses, lentiviruses, retroviruses and non-viral vectors for use in research projects. Its state-of-the-art research materials – including helper cell lines, viral vectors, and non-viral vectors – are freely available to investigators.
The facility also provides comprehensive production and analysis services. For example, the Core can construct a vector for an investigator's designated purpose, produce and purify the corresponding recombinant vector in quantity, and/or evaluate the quality of the material.
The Core also provides instruction and consultation during all phases of a research project related to gene transfer in vitro or in vivo.
Initial requests for services should be discussed with the Director.
- To provide a Core Laboratory for the construction, purification and characterization of recombinant vectors containing transgenes for use as in vitro and in vivo gene transfer reagents. These systems include both non-viral (expression plasmid) and viral (recombinant retrovirus, lentivirus and adenovirus) technologies.
- To serve as a repository and in-house distribution system for the Ultimate™ ORF clone collection purchased from Life Technologies Inc.
- To provide access to the technology associated with the use of gene transfer systems to investigators in an efficient and user-friendly manner.