Analysis

CRISPR

Clustered Regularly Interspaced Short Palindromic Repeats is a part of adaptive immune system and is used by many bacterial species. For example in case of bacteriophage, the sequences from viral genome is integrated into the bacterial genome. Bacteria uses the antisense RNA generated during the next attack of bacteriophage as the memory signature as a defense mechanism.

CRISPR-Cas genome editing techniuqe has borrowed the above mentioned concept from bacteria and archaea.The development of CRISPR-Cas systems has enabled the scientists to make changes (addition, removal or alteration at desired location of the genome) in the genome of organisms. The methodology has greatly enriched our understanding in the field of human diseases, food, agriculture etc. Please see the article for more information on CRISPR

Here is the timeline of the development of CRISPR as the genome editing technique.

Below is the flowchart showing various steps in CRISPR screen methodology.

 

crispr screening
Flow chart showing crispr methodology

(Source: Joung, J., Konermann, S., Gootenberg, J.S., Abudayyeh, O.O., Platt, R.J., Brigham, M.D., Sanjana, N.E., Zhang, F., 2017. Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening. Nat. Protoc. 12, 828–863.)

We here at the McDermott Center Bioinformatics Lab, help researchers with the experimental design and bioinformatics analysis of the screening results. Please contact us for discussing your bioinformatics queries regarding the analysis of the screening results.