David Chuang, PhD

Professor
Biochemistry, Internal Medicine
Graduate Program: Biological Chemistry

Contact Information

UT Southwestern Medical Center
5323 Harry Hines Boulevard
Dallas, Texas 75390

Office Phone: 214-648-3338
Office Fax: 214-648-8856

david.chuang@utsouthwestern.edu

Education

Graduate SchoolUtah State University (1970)
Graduate SchoolNational Taiwan University - Taiwan (1964)

Research Interests

Chaperone-mediated biogenesis of mitochondrial multi-enzyme complexes.
Molecular genetic and biochemical basis of maple syrup urine disease.
Structure and protein-protein interactions of mitochondrial multienzyme complexes.

Publications

Featured
Encapsulation of an 86-kDa Assembly Intermediate inside the Cavities of GroEL and Its Single-ring Variant SR1 by GroES.

Song, J..-L., Li, J., Huang, Y.-S., and Chuang, D.T. , J. Biol. Chem. , 2003; (278):2515-2521

Featured
Solution structure of the lipoic acid-bearing domain of human mitochondrial branched-chain (alpha)-ketoacid dehydrogenase complex.

Chang, C.-F., Chous, H.-T., Chuang, J.L., Chuang, D.T., and Huang, T.-H. , J. Biol. Chem. , 2002; (277):15865-15873

Featured
Structure of rat BCKD kinase: nucleotide-induced domain communication in a mitochondrial protein kinase.

Machius, M., Chuang, J.L., Wynn, R.M., Tomchick, D.R., and Chuang, D.T. , Proc. Natl. Acad. Sci. USA , 2001; (98):11218-11223

Featured
Natural osmolyte trimethylamine N-oxide corrects assembly defects of mutant branched-chain (alpha)-ketoacid dehydrogenase in maple syrup urine disease.

Song, J.-L., and Chuang, D.T. , J. Biol. Chem. , 2001; (276):40241-40246

Featured
Crystal structure of human branched-chain (alpha)-ketoacid dehydrogenase and molecular basis of multienzyme complex deficiency in maple syrup urine disease.

Aevarsson, A., Chuang, J.L., Wynn, R.M., Turley, S., Chuang, D.T., and Hol, W.G.H. , Structure , 2000; (8):277-291

Crystal structure of pyruvate dehydrogenase kinase 3 bound to lipoyl domain 2 of the human pyruvate dehydrogenase complex.

Kato, M., Chuang, J.L., Tso, S.-C., Wynn, R.M., and Chuang, D.T. , EMBO J. , 2005; (24):1763-1774

Crystal structure of human dihydrolipoamide dehydrogenase: NAD+/NADH binding and the structural basis of disease-causing mutations.

Brautigam, C. A., Chuang, J. L., Tomchick, D. R., Machius, M. and Chuang, D. T. , J. Mol. Biol. , 2005; (350):543-552

Molecular mechanism for regulation of the human mitochondrial branched-chain a-ketoacid dehydrogenase complex by phosphorylation.

Wynn, R.M., Kato, M., Machius, M., Chuang J.L., Li J, Tomchick D.R., and Chuang, D.T. , Structure , 2004; (12):2185-2196

Seeing GroEL at 6 ? resolution by single particle electron cryomicroscopy.

Ludtke, S., Chen, D., Song, J.-L., Chuang, D.T., and Chiu, W. , Structure , 2004; (12):1129-1136

Crosstalk between thiamin diphosphate binding and phosphorylation loop conformation in human branched-chain a-ketoacid decarboxylase/dehydrogenase.

Li, J., Wynn, R. M., Machius, M., Chuang, J. L., Karthikeyan, S., Tomchick, D. R., and Chuang, D. T. , J. Biol. Chem. , 2004; (279):32968-32978

Honors/Awards

VA Administration Central Ofc-Washington DC

Associate Career Scientist Award (1988)

VA Medical Center-Cleveland OH

Outstanding Performance Awards (1987)

VA Medical Center-Cleveland OH

Outstanding Performance Awards (1986)

VA Medical Center-Cleveland OH

Outstanding Performance Awards (1985)

Professional Associations/Affiliations

American Association for the Advancement of Science

American Society for Biochemistry and Molecular Biology

American Society for Human Genetics

Society of Chinese Bioscientists in America