Intermediary metabolism is the complex process by which nutrients are transformed into cellular building blocks, redistributed to specific cellular fuels, converted to storage molecules or oxidized to provide energy. Disorders of intermediary metabolism are the basis of many diseases, so techniques that would allow quantitative measures of flux of molecules through these metabolic pathways are important to basic and clinical science. Nuclear Magnetic Resonance (NMR) spectroscopy in conjunction with stable isotope (non-radioactive) tracers is a powerful technique for understanding intermediary metabolism. NMR is especially informative about the chemical nature of an atom (and its neighboring atoms) in a molecule and thus provides enormous insight into the metabolic fate of isotope labeled tracers. Administration of stable isotope tracers, containing carbon-13 or deuterium, in humans or animals followed by NMR analysis of tissue or fluid extracts reveals the metabolic flux of a number of biochemical pathways. My interest is in using this technology to study hepatic energy production and gluconeogenesis in the liver and kidney. My lab studies these pathways in mouse models having specific defects in hepatic energy and glucose metabolism and has translated the information gained from animal studies towards the understanding of human physiology by partnering with clinicians who have human subjects with abnormal metabolic features such as obesity and diabetes. This powerful cross-disciplinary approach unites my interests in metabolism with molecular biologists and clinicians, providing an increasingly integrated picture of the physiology of metabolic diseases.
RESEARCH INTERESTS
Biological NMR methods
Intermediary metabolism: Elucidation of metabolic pathways
In Vivo and Ex Vivo isotope tracer techniques
Metabolism in animal models of diabetes and obesity
RECENT PUBLICATIONS
Shawn C Burgess, Merrill Nuss, Bernard R Landau, A Dean Sherry and Craig R Malloy, "Comparison of 2H2O Measured Gluconeogenesis Determined by Both NMR and MS" Analytical Biochemistry, 318:321-324, 2003
Inagaki T, Dutchak P, Zhao G, Ding X, Gautron L, Parameswara V, Li Y, Goetz R, Mohammad M, Esser V, Elmquist JK, Gerard RD, Burgess SC, Hammer RE, Mangelsdorf DJ, Kliewer SA, "Regulation of the Fasting Response by PPARa-Mediated Induction of Fibroblast Growth Factor 21" Cell Metabolism, IN PRESS 2007
Browning JD, Davis J, Saboorian MH, and Burgess SC, "A low-carbohydrate diet rapidly and dramatically reduces intrahepatic triglyceride content. Hepatology" Hepatology, 44:487-488, 2006
Natasha Hausler, Matthew Merritt, Charles Storey, Angela Milde, A. Dean Sherry Craig R. Malloy and SC Burgess, "Effects of Insulin and Cytosolic Redox State on Glucose Production Pathways in the Isolated Perfused Mouse Liver by Integrated 2H and 13C NMR" Biochemical Journal, 395:663, 2006
SIGNIFICANT PUBLICATIONS
Danhong Lu, Hindrik Mulder, Piyu Zhao, Shawn C Burgess, Mette V Jensen, Svetlana Kamzolova, Christopher B Newgard and A Dean Sherry, "13C NMR Isotopomer Analysis Reveals a Connection Between Pyruvate Cycling and Glucose-Stimulated Insulin Secretion (GSIS)" PNAS, 99:2708-2713, 2002
Pengxiang She, Shawn C. Burgess, Masakazu Shiota, Paul Flakoll, E Patrick Donahue, Craig R Malloy, A Dean Sherry, and Mark A Magnuson, "Mechanisms by which liver-specific PEPCK knockout mice preserve euglycemia during starvation" Diabetes, 52:1649-1654, 2003
Burgess SC, Hausler N, Merritt M, Jeffrey FMH, Storey C, Milde A, Koshy S, Lindner J, Magnuson MA, Malloy CR, and Sherry AD., "Impaired Tricarboxylic Acid Cycle Activity in Mouse Livers Lacking Cytosolic Phosphoenolpyruvate Carboxykinase" Journal of Biological Chemistry, 279:48941-48949, December 2004
Burgess SC, Leone TC, Wende AR, Croce MA, Chen Z, Sherry AD, Malloy CR, and Finck BN, "Diminished hepatic gluconeogenesis via defects in tricarboxylic acid cycle flux in peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC-1alpha)-deficient mice" Journal of Biological Chemistry, 281:19000-19008, December 2004
34. Burgess SC, He T, Yan Z, Lindner J, Sherry AD, Malloy CR, Browning JD, Magnuson MA, "Cytosolic Phosphoenolpyruvate Carboxykinase Does Not Solely Control the Rate of Hepatic Gluconeogenesis in the Intact Mouse Liver" Cell Metabolism, 5:313-320, April 2007
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