The application of nuclear magnetic resonance (NMR) as a tool for measuring intermediary metabolism depends upon high NMR sensitivity for carbon-13 and deuterium nuclei. The sensitivity is governed by the applied magnetic field strength and the efficiency of the detection circuit. Recent data acquired using dynamic nuclear polarization (DNP) to enhance the magnetic resonance signal shows an increase in signal to noise of a factor of 10000 or more. Sensitivities of this magnitude would fundamentally change the types of problems we can approach with stable isotope methods. We are beginning a research program focused on using DNP for NMR analysis of metabolism. Our first papers show that hyperpolarization does provide unique opportunities to monitor enzyme catalyzed reactions with extremely high time resolution (1 second or less). These results represent a quantum leap in the power of NMR to study metabolism.
RESEARCH INTERESTS
Magnetic Resonance
Hyperpolarization for NMR sensitivity enhancement
Metabolomics and Efficient NMR Data Reduction
RECENT PUBLICATIONS
Merritt, Matthew; Harrison, Crystal; Storey, Charles; Jeffrey, F. Mark; Sherry, A. Dean; Malloy, Craig R., "Hyperpolarized 13C allows a direct measure of flux through a single enzyme-catalyzed step by NMR" Proceedings of the National Academy of Sciences, 104/50:19773-19777, 2007
Merritt, Matthew E.; Harrison, Crystal; Storey, Charles; Jeffrey, F. Mark; Sherry, A. Dean; Malloy, Craig R, "Inhibition of Carbohydrate Oxidation During the First Minute of Reperfusion After Brief Ischemia: NMR Detection of Hyperpolarized 13CO2 and H13CO3-." Magnetic Resonance in Medicine, 60/5:1029-1036, 2008
SIGNIFICANT PUBLICATIONS
Merritt, M., Bretthorst, GL., Burgess, S., Jeffrey, FMH., Sherry, AD., Malloy, C.,, "TCA cycle turnover and serum glucose sources by automated Bayesian analysis of NMR spectra." Magnetic Resonance in Medicine, 50(4):659-663, 2003
Woessner, D., Zhang, S., Merritt, M., Sherry, A. D., "Numerical solution of the Bloch equations provides insights into the optimum design of PARACEST agents for MRI." Magnetic Resonance in Medicine, 53(4):790-799, 2005
Merritt, Matthew E.; Harrison, Crystal; Kovacs, Zoltan; Kshirsagar, Preeti; Malloy, Craig R.; Sherry, A. Dean, "Hyperpolarized 89Y Offers the Potential of Direct Imaging of Metal Ions in Biological Systems by Magnetic Resonance" Journal of the American Chemical Society, 129/43:12942-12943, 2007
Merritt, Matthew E.; Harrison, Crystal; Mander, William; Malloy, Craig R.; Sherry, A. Dean, "Dipolar cross-relaxation modulates signal amplitudes in the 1H NMR spectrum of hyperpolarized [13C]formate" Journal of Magnetic Resonance, 189/2:280-285, 2007
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