Facilities and Equipment

Facilities

The Molecular Radiation Biology division has nearly 10,000 square feet of dedicated space conveniently located on one floor in a modern facility. At our disposal is a dedicated laboratory coordinator who assists in monitoring core equipment. The division currently maintains more than 100 PC and Macintosh computer systems. 

Within our dedicated space we have 10 research labs, five tissue culture rooms for a total of 11 tissue culture hoods. Each principle investigator is provided a separate office space. In addition we have two rooms dedicated for microscope use. One room houses a Zeiss LSM 510 Confocal Microscope and a Zeiss-Axiovert Microscope, while the other room houses two Zeiss and one Olympus fluorescent microscopes. All microscopes are equipped with dedicated cameras and computers.

In addition to our 10,000 square feet of dedicated space, we share eight rooms with two additional labs outside RadOnc Dept. Those rooms are:

  • Temperature specific rooms: -20ºC room, 4ºC room, 37ºC room
  • Equipment gallery housing three centrifuges, three cryo freezers, and 11 ultralow freezers
  • Additional room housing a state-of-the-art CO2 system
  • Room housing three autoclaves, one dishwasher, and one oven
  • Darkroom

Equipment

Small Animal Cabinet X-Ray Irradiator

X-RAD 320, a self-contained X-ray irradiation system for the use of animal studies, with maximum 320 kV output. A fee of $50/hour will be charged to users from outside of the department. For more information, please contact Debabrata Saha, Ph.D. Email

Keyence BZ-X700

Keyence BZ-X700

An all-in-one fluorescent microscope for automated high-content imaging in fixed and live cells. The system also equips with analytic programs for fast and reliable image analyses.

Seahorse XFe24 Analyzer

Seahorse XFe24

Measures OCR and ECAR of live cells in a 24-well plate format, which provides key indicators of mitochondrial respiration and glycolysis and a systems-level view of cellular metabolic function in cultured cells, islets, and ex vivo samples.

FlowSight® Imaging Flow Cytometer

FlowSight

This flow cytometer produces up to 12 images of each cell.

GE Healthecare Life Sciences IN Cell Analyzer 2000

GE IN Cell 2000

A flexible, modular, lamp-based cellular and subcellular imaging system for automated high-content imaging in fixed and live cells. Provides high-quality images, across a broad range of cellular and subcellular assay types.

HypOxygen H35 HypOxystation and Whitley i2 Instrument Workstation

HypOxygen H35 & i2

Consists of both cell incubator and workstation to allow a controlled environment for cell manipulation under normoxic, hypoxic, and anoxic conditions.

GE Healthcare Typhoon 9410 Scanner

Typhoon 9410

Imaging via storage phosphor auto-radiography, chemiluminescence, or excited fluorescence.

Zeiss LSM 510 META Confocal Microscope

Equipped with a Zeiss ConfoCor 3 FSC module, an external MicroPoint Nitrogen Laser, and a stage heater for live cell imaging analysis. It is used to investigate the temporal and kinetics of DNA damage sensing and signaling processes in live cells.

Zeiss AxioImager M2 Microscope

Configured to perform fluorescence microscopy as well as differential-interference contrast and phase-contrast microscopy. Images may be acquired via a Zeiss AxioCam MRm B/W camera or a AxioCam ICc color camera.

Zeiss Axiovert 200 and Axiovert 200M Inverted Research-Grade Microscope

Configured to perform fluorescence microscopy as well as differential-interference contrast and phase-contrast microscopy. Images may be acquired via a Zeiss AxioCam HRC color CCD camera.

NanoDrop 2000 UV-Vis Spectrophotometer

NanoDrop 2000

For measuring peptides, DNA, RNA, or protein concentrations with minimum sample volume.

Ultrospec 3300 Spectrophotometer

To measure the amount of a chemical substance absorbs light by passing a beam of light through the sample. It can read a wavelength range of 190 to 1,100 nm.

Leica CM3050S Motorized Cryostat and SM2000R Sliding Microtome

Tissue sectioning.

Bio-Rad CFX90 Touch™ Real-Time PCR Detection System

Bio-Rad CFX90 Toch Real-Time PCR

Six-channel real-time PCR instruments with precise temperature control to deliver sensitive, reliable detection for singleplex or multiplex reactions.

Bio-Rad T100 Thermal Cycler

Bio-Rad T100

For PCR, gene cloning and analysis, gene expression analysis, mutational analysis, and cycle sequencing.

Bio-Rad QX200 Droplet Digital PCR System

Provides quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications.

Polarstar Optima Microplate Reader

Polarstar Optima Microplate Reader

A fully automated microplate based multi-detection reader incorporates five different measurement principles.

ProteinSimple FluorChem M Multifluor System

ProteinSimple FluorChem M

For chemiluminescent imaging.

ProteinSimple Wes

ProteinSimple Wes

The instrument lets the users separate and analyze proteins by size from 2 kDa to 440 kDa. It can run up to 25 samples in just three hours.

ProteinSimple NanoPro 1000 Simple Western Charge Assays

Provides a protein/phospho-protein quantitative assay. This instrument is also capable of analyzing drug actions in fine needle aspirates (FNAs) taken from xenografts, and requires as few as 25 cells per assay.

Custom BioGenic Systems V-3000A and Thermo CryoPlus 3 (x2) Liquid Nitrogen Freezers

For frozen sample storage at liquid nitrogen temperature.

Nucleofector II Device

For DNA transfection into cells, up to 90 percent transfection efficiency.

Colcount Automated Colony Counter

For clonogenic survival analysis.

Z1 Analyzer and Z2 Analyzer (x2)

For cell culture count including size distribution of the cell population.

AKTA pure Chromatography System

For protein purification and high-resolution analysis.

Thermo Scientific™ CytoSpin™ 4 Cytocentrifuge

Designed for thin-layer cell preparation.

SORVALL Discovery 90SE Ultracentrifuge

SORVALL RC-6 Superspeed Refrigerated Centrifuge