DNA Next Generation Sequencing Facility
Pricing and Sample Requirements

Pricing is based on a cost-recovery model, and will vary depending on the experiment and data requirements. Please email Vanessa Schmid (vanessa.schmid@utsouthwestern.edu) for an estimate for your project.

Pricing includes primary and basic secondary computational analyses.

Rapid Runs are also available for sequencing lengths with 50-150 bp; ask for details.

Typical charges for samples in which the sequencing core makes the libraries are:

ProcedureCoverage (X)
or # reads (M)
Sample
amount
TypeRead length (bp)Samples
/lane
Price
ChIP 25M 10 ng Single-end 50 6 $375
Exome (Human) 50X 2 μg Paired-end 100 6 $900
Exome (Mouse) 50X 2 μg Paired-end 100 6 $1,200
mRNA, ss* 40M 5 μg Single-end 50 4 $475
mRNA, ss 40M 5 μg Paired-end 100 4 $725
Small RNA 25M 2 μg Single-end 50 6 $450
Whole Transcriptome, ss 40M 2 μg Single-end 50 4 $540
Whole Transcriptome, ss 40M 2 μg Paired-end 100 4 $790
Whole Genome 40X 2 μg Paired-end 100 0.25** $8,590

*Strand-specific.

**Whole genome sequencing at 50X coverage requires sequencing of a single library in four lanes.

Instructions for Submitting NGS Samples 

Submit samples in 1.5 ml Lo-bind microfuge tubes, labeled clearly with order number, submission date, and sample identity. Sample quantification is required prior to submission.

NOTE: Concentrations determined by NanoDrop are very unreliable. Please quantify the DNA and RNA concentrations using fluorometry. If you cannot do fluorometry, please send us 2X the minimum amount, as determined by NanoDrop.

ProcedureMinimum
Quantity
RNA Integrity
(RIN) Score
Concentration

RNA-Seq Samples
mRNA Sequencing

RNA needs to be treated with DNAse prior to quantification 

μg > 8.0 ~100 ng / μl

Whole Transcriptome

RNA needs to be treated with DNAse prior to quantification 

μg > 8.0 ~100 ng / μl
Illumina Small RNA Seq μg > 8.0 ~200 np / μl

ChIP-Seq Samples

DNA should be sheared to 200 - 600 bp 

5 ng for the ChIP DNA Sample

50 ng for the input DNA Sample 

DNA Integrity: Provide gel image of your input DNA with size standards

Exome Sequencing

μg

DNA Integrity: Provide gel image of your input DNA with size standards

~100 ng / μl
Whole Genome Sequencing

μg

DNA Integrity: Provide gel image of your input DNA with size standards

~100 ng / μl

Submission of Prepared Library Requirements

Before making your libraries, please make sure your barcodes are compatible with Illumina Standards (for details, see page 12 of Illumina's TruSeq® Sample Preparation Pooling Guide). We discourage the use of investigator-designed barcodes.

Minimum concentration: 2 nM (measured by fluorometry)
Minimum volume: 15 μ

If your samples do not meet these requirements, you will need to pool your samples into lane pools at 2 nM. We will need 20 μl of this pool.

Unfortunately we cannot offer any guaranteed cluster yield or quality for libraries prepared outside of the McDermott Center NGS Core, but we are happy to answer any questions you have regarding library preparation.